Bio-rad ChromLab™ Software Manuel d'utilisateur télécharger pdf (Page 142)

5 | Plumbing and Priming the NGC Chromatography Systems

140 | NGC Chromatography Systems and ChromLab Software

To purge the buffer inlet valves

1. Open the System Pump dialog box and select Priming on the Mode dropdown

list.

Note: When in Priming mode, the sample inject valve switches the system

pump flow to waste (W2). After the pr

ocess is completed, the sample inject

valve switches the system pump to manually load the column through the

loop.

2. Select the first buffer port to purge, for example Buffer A 1.

3. (Optional) Change the default flow rate.

The default flow rate is set to 10 ml/min.

4. Click Start.

5. Repeat steps 2–4 for the remaining buffer inlet ports.

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Bio-rad ChromLab™ Software Manuel d'utilisateur Page 142