Bio-rad BioLogic Sample Loading Loops Manuel d'utilisateur

0510 15 202501015 20 25 30 35 40 45 50 55 60 65 70 75 80 85 905DynaLoop™25andDynaLoop 90InstructionManualCatalog Numbers750-0451 (25 ml)750-0452 (90 m

3. Connect the sample end of the DynaLoop to port 3 of the injection valve (Figure 6).4. Connect the buffer end of the DynaLoop to port 6 of the injec

Electrical Connections (Model EP-1 Econo Pump)1. Turn off the BioLogic DuoFlow controller and workstation.2. Connect the EP-1 Econo pump to the BioLog

6. Some air may still be trapped in the sample chamber of the DynaLoop. If so, switch theinjection valve to the INJECT position and again empty the sa

2. Connect the Econo gradient pump inlet tube to a reservoir of buffer. Switch the injectionvalve to the INJECT position. Set the BioLogic DuoFlow flo

Load Sample Manually (via a syringe)1. With the injection valve in the INJECT position, allow the sliding seal to contact thesample end fitting. Set t

d. In the Inject Sample area of the window, select the Injection Buffers, the buffercomposition, the flow rate of the BioLogic DuoFlow workstation pum

4.2 Cleaning and StorageRefer to Figure 9 as you follow these instructions.Fig. 9. DynaLoop cleaning.Cleaning and StorageWith the DynaLoop in the flu

Reassembly1. Hold the body of the sliding seal assembly in one hand. Insert, in order, the rubber ball,the check valve guide, and spring into the hole

Caution: Do not pry against the black plastic lip on the end fitting that holds the O-ring.The connector tip may break, causing the DynaLoop to leak.

Section 5Appendix A Replacement PartsTable 2. DynaLoop Replacement PartsProductDescription Catalog NumberDynaLoop Parts Kit, includes end cap O-rings,

Life ScienceGroup00-0000 0000 Sig 0308Bulletin 0000 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Australi

Table of ContentsPageSection 1 Introduction...11.1 DynaLoop Sample

List of FiguresPageFigure 1 DynaLoop 25 and DynaLoop 90 Sample Loops ...1Figure 2 Sliding Seal Assembly (Load Phase) ...

Section 1Introduction1.1 DynaLoop Sample Loop DescriptionThe DynaLoop sample loop is a large-volume sample injection loop for use with theBioLogic Duo

2Load phase With the AVR7-3 injection valve in the LOAD position, sample is injectedinto the sample chamber end of the DynaLoop via port 3 on the inje

Fig. 3. Flush phase. (Check valve is open.)Section 2Installation2.1 UnpackingThe DynaLoop is shipped fully assembled as shown in Figure 1.List of comp

Important Considerations• Always follow good laboratory practices when handling solvents.• Use buffers and solvents that are chemically clean and free

Make Connections1. To make a collet/nut connection at the end of the DynaLoop, slide a collet/nut fittingand ferrule over the end of the cut tubing. M