Bio-rad ProteinChip Qualification and Calibration Kits Manuel d'utilisateur télécharger pdf (Page 31)

12. Click OK. The spectrum flag changes from yellow to green for all 8 spectra, once calibration

is complete.

13. Export the data following the instructions in Appendix C and selecting the parameters for

export in the following order:

• Array bar code

• Spot name

• Substance mass

• Intensity

• Resolution

• S/N

• Peak #

14. Paste the data into the ProteinChip SELDI OQ form, data sheet “Test 4 Mass Calibration”.

Paste the data into cell V41.

15. In the Internal Table, right-click on cell B21 and select Refresh Data.

16. The spreadsheet indicates if the test passed or failed. The test passes if the following

specifications are achieved:

• Peptide average mass within 0.01% of calibrant mass

• % Standard deviation (pooled CV) of <0.01

17. If the test fails, run maintenance procedure 1 (HV conditioning) and repeat the test. Contact

technical support if the test fails a second time.

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Bio-rad ProteinChip Qualification and Calibration Kits Manuel d'utilisateur Page 31