Bio-rad ProteinChip Qualification and Calibration Kits Manuel d'utilisateur

ProteinChip®OQ KitInstruction Manual andDocumentationCatalog #C70-00080For technical support call your local Bio-Rad office, or in the US, call 1-800-

Step 4Create a StandardMass CalibrationRoutine76. In the Explorer pane of ProteinChip data manager software, select Projectsand click on any spectrum

83. In the Calibration protocol name field, enter OQ Mass Cal.4. Select [Arg8]-Vasopressin (1084.247) from the drop-down list, and click Add(keep the

2.3 OQ Maintenance Procedures 2.3.1 Maintenance Procedure 1 –– High Voltage (HV) Conditioning This procedure helps to decontaminate the surfaces of t

1. Insert the ProteinChip detector calibration array into the instrument.Note: For the Enterprise Edition instrument, place the array into slot 1 of a

7. The time required to complete this procedure is variable, and it may require up to severalhours as the instrument continually collects data of a sp

2.4 OQ Tests2.4.1 Test 1 –– Detector Sensitivity This test uses the ProteinChip detector qualification array to measure the signal-to-noise ratio(S/

6. Plot the peaks with noise set to 12,000 Da:a. Select all 8 spectra.b. Click on the data analysis icon . The Analysis Settings dialog opens.c. Open

7. Plot all 8 spectra and select the IgG peak using the peak selection tool.Note: Spots with high concentrations (spots A, C, E, and G) alternate with

2.4.2 Test 2 — Mass Drift and Resolution 5.96 kD This test uses the ProteinChip peptide standard array to measure the mass drift and resolutionof ins

6. Run the same protocol on partition “n” of 4 for all 8 spots. Record the partition number on the usage chart provided with the product packaging.7.

NoticesNo part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, record

8. Plot the peaks with the filtering option turned off:a. Select all 8 spectra. (Do not select the first laser optimization spectrum.)b. Click the ana

9. Plot all 8 spectra. Using the peak selection tool, select the insulin peak (5.963 kD) in all spectra.10. Export the data following the instructions

2.4.3 Test 3 –– Resolution at 1 kDThis test uses the ProteinChip peptide standard array to measure the resolution of Arg-8-vasopressin.To do this, a

8. Plot the peaks with the filtering option turned off:a. Select all 8 spectra.b. Click the data analysis icon . The Analysis Settings dialog opens.c

9. Plot all 8 spectra (do not select the first optimization spectrum). Using the peak selectiontool, select the Arg-8-vasopressin (1,084.247 Da) peak.

2.4.4 Test 4 — Mass Accuracy This test uses the ProteinChip peptide standard array to test the mass accuracy of the system’sinternal and external cal

7. Plot the peaks with the filtering option turned off:a. Select all 8 spectra.b. Click the data analysis icon . The Analysis Settings dialog opens.c

Note: To ensure correct placement of the peak marker, zoom in on each peak during selection.Though some plots appear acceptable when zoomed out, zoomi

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9. Zoom in and check that all 7 peaks in all 8 spectra are marked correctly. Failure to do somay result in incorrect results. Close the plot.Mass Accu

Table of ContentsChapter 1 Introduction ...11.1 Product and P

9. In the External Table, right-click on cell B3 and select Refresh Data.10. The spreadsheet indicates if the test passed or failed. The test passes i

12. Click OK. The spectrum flag changes from yellow to green for all 8 spectra, once calibrationis complete.13. Export the data following the instruct

Appendix A2910010680A.qxp 8/17/2007 1:59 PM Page 29

Appendix BProtocol Acceptance FormBio-Rad Laboratories recommends that the operational qualification (OQ) protocols be performedin total on a regular

Appendix CData Export ProcedureUse this procedure to export the data obtained during testing to the Microsoft Excel spreadsheetprovided with the kit (

Life ScienceGroup00-0000 0000 Sig 1106Bulletin 0000 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra

Chapter 1IntroductionQualification of analytical instruments is a formal process of documenting that an instrument isfit for its intended use and that

Completion of each maintenance procedure and test is tracked using the ProteinChip SELDI OQform, a Microsoft Excel spreadsheet supplied on the CD. Tes

Table 1. Summary of procedures and tests (continued).RReeccoommmmeennddeedd CCoonnssuummaabbllee EEssttiimmaatteedd TTiimmee TTeesstt FFrreeqquuee

Chapter 2Use of the ProteinChip OQ Kit2.1 OverviewThis chapter outlines the protocols used to perform the procedures and tests that comprise theProte

2.2 File and Protocol SetupComplete this procedure the first time you run the ProteinChip OQ kit. This procedure sets upthe file structure necessary

Step 3Set Up theHigh Voltage (HV)Conditioning Routine61. Insert a blank ProteinChip array into the reader. If blank arrays are notavailable, use expir