Bio-rad Comparative Proteomics Kit II: Western Blot Module Manuel d'utilisateur

25Quick GuideQUICK GUIDE1mlComparative Proteomics Kit II:Western Blot Module – QuickGuideLesson 1 Quick Guide1 Label one 1.5 ml fliptop micro tube for

5. Slide gel cassette, buffer dam, andelectrode assembly into the clampingframe.6. Press down the electrode assembly whileclosing the two cam levers o

27Quick GuideQUICK GUIDE11. Heat fish samples and actin and myosinstandard to 95°C for 2–5 min.12. Load your gel:Lane Volume Sample1 & 2 empty Emp

28QUICK GUIDEQuick GuideBanana PlugsAnode Banana Plug(Red)Notch onU-Shaped GasketGel SandwichPressure PlateCamsMini TankInnerChamberAssemblyClampingFr

Lesson 3 Quick Guide1. Using a ruler, chop the top and bottom offthe gel. 2. Equilibrate the gel in blotting buffer for 15 minutes on a rocking pla

QUICK GUIDEQuick Guide6. Set up the Mini Trans-Blot module with theblack side of the cassette next to the blackside of the Mini Trans-Blot module. Ad

Fig. 5. Assembly of the Mini Trans-Blot cell.31QUICK GUIDEQuick GuideElectrodemoduleLidFiber padBlotting paperMembraneGelBlotting paperFiber padGel h

Lesson 4 Quick Guide1. If not blocked overnight, immerse membrane in 25 ml blocking solution for 15 minutes to 2 hours at room tempera-ture on a rock

Life ScienceGroup06-0372 0606 Sig 1205Bulletin 5478 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra