Bio-rad ProteinChip Data Manager Software Manuel d'utilisateur Page 1

ProteinChip®SELDI System:Reader GuidePersonal Edition and Enterprise Editon

Safety Precautions Page 5 Fig. 4: Safety symbols indicating high voltage inside the ProteinChip SELDI system cabinet. These labels are located on t

Page 6 ProteinChip SELDI System: Reader Guide glasses must have an optical density of >5 at 337 nm. Care should be taken to avoid specular reflec

Installing the ProteinChip SELDI Reader Page 7 Ventilation The ProteinChip SELDI reader requires 3.0 cm of space at the rear and 3.0 cm above the ve

Page 8 ProteinChip SELDI System: Reader Guide Fig. 6: The ProteinChip SELDI reader, Personal Edition, on its shipping pallet. 3. Inspect the reade

Setting Up the ProteinChip SELDI System Page 9 Description: Sinergy 1KVA UPS, 120 V AC input/output, 60 Hz line frequency. 5-minute backup time.

Page 10 ProteinChip SELDI System: Reader Guide Launch the software 1. Locate the ProteinChip data manager client software shortcut on the desktop a

Setting Up the ProteinChip SELDI System Page 11 Fig. 8: The New Instrument dialog box.3. Enter a name for the reader into the Name field. This can be a

Page 12 ProteinChip SELDI System: Reader GuideConnect to the reader using ProteinChip data manager client software1. After the reader profile has been

Operating the ProteinChip SELDI Reader Page 13 3. In ProteinChip data manager client software, connect to the reader by clicking its name in the Explo

Page 14 ProteinChip SELDI System: Reader GuideMulticassette Autoloader (Enterprise Edition)The ProteinChip SELDI system Enterprise Edition comes with

Page i Bio-Rad Laboratories, Inc.Notice to Purchaser: Limited LicenseSurface Enhanced Laser Desorption/Ionization (“SELDI”) is a patented process. To

Operating the ProteinChip SELDI Reader Page 15 Fig. 12: Sensor status before beginning operation.Manual ModeManual Mode allows you to manually set dat

Page 16 ProteinChip SELDI System: Reader GuideFig. 13: Collecting data in Manual Mode (Enterprise Edition).Protocol ModeProtocol Mode allows you to a

Operating the ProteinChip SELDI Reader Page 17 Fig. 14: Collecting data in Protocol Mode (Enterprise Edition).Auto Mode (Enterprise Edition only)Auto

Page 18 ProteinChip SELDI System: Reader GuideFig. 15: Collecting data in Auto Mode (Enterprise Edition).Shutting Down the ProteinChipSELDI ReaderTo

Basic Maintenance Page 19 2. Wait for the System Active light on the lower right side of the reader to go out, then turn off the reader power.3. Remov

Page 20 ProteinChip SELDI System: Reader GuideFig. 18: Pawl drive front.7. Gently pull the pawl drive assembly back and up to clear it from the load

Basic Maintenance Page 21 Fig. 19: Pawl drive assembly, pulled back from front plate.8. Using the pins on the front, unscrew the load lock door from i

Page 22 ProteinChip SELDI System: Reader Guide10. Replace the load lock door by screwing it clockwise back on to the post. 11. Tighten the door until

Warranty and Service Agreements Page 23 14. Replace the thumb screws on the front of the pawl drive. 15. Manually open and close the load lock door to

Page 24 ProteinChip SELDI System: Reader GuideGetting Technical AssistanceFor technical service, call your local Bio-rad office, or in the US, call 1-

Page ii ProteinChip SELDI System: Reader Guide

Moving the ProteinChip SELDI System Reader Page 25 Appendix A: Networking the ProteinChip SELDI SystemThe ProteinChip SELDI system requires a computer

Page 26 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideTwo methods can be used to network the ProteinChip SELDI system

Networking the ProteinChip SELDI System Using DHCP Page 27 Fig. 23: The reader web page.4. Next, click the Software link. If the first number in the Cu

Page 28 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 24: Checking the reader software version.Set the Reader to

Networking the ProteinChip SELDI System Using DHCP Page 29 Fig. 25: The reader web page.4. Click on the Settings link. The Network Settings page will

Page 30 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 26: The Network Settings web page.5. Click the Obtain netwo

Networking the ProteinChip SELDI System Using DHCP Page 31 7. Record the reader's hostname. The format of the hostname is pcs4000-xxxx, where xxx

Page 32 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 28: The Internet Protocol (TCP/IP) Properties dialog box.2.

Networking the ProteinChip SELDI System Using DHCP Page 33 Fig. 29: The Servers dialog box.3. Highlight the alias for the current server (not the loca

Page 34 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideObtain a New License File (If Necessary)After changing the serve

Page iii Table of Contents Introduction 1Specifications 1ProteinChip SELDI Reader 1Mass Analysis Range 1Sensitivity 1Mass Resolving Power 2Mas

Networking the ProteinChip SELDI System Using DHCP Page 35 2. Click on the Instruments node in the Explorer tree. A table of the available instrument

Page 36 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 33: The Instrument Properties dialog box.4. Enter the hostn

Networking the ProteinChip SELDI System Using DHCP Page 37 Fig. 34: The Home tab of the ProteinChip data manager software.Edit the Reader OptionsAfter

Page 38 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader Guide2. Enter the hostname of the server into the Data Manager Server

Networking the ProteinChip SELDI System Using Static IP Addresses Page 39 If you need to configure a separate computer to communicate with the reader,

Page 40 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 37: The Network Settings web page.5. Enter the network sett

Networking the ProteinChip SELDI System Using Static IP Addresses Page 41 Assign a Static IP to the Server ComputerA second static IP address must be

Page 42 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideDHCP settings to take effect. If the computer receives a reply t

Networking the ProteinChip SELDI System Using Static IP Addresses Page 43 Fig. 40: The Edit Server dialog box.4. In the Edit Server dialog box, enter

Page 44 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 41: Errors produced when the server needs a new license file

Page ivProteinChip SELDI System: Reader Guide Basic Maintenance 18Load Lock Door Cleaning for Enterprise Edition Instruments 18Load Lock Door Clean

Networking the ProteinChip SELDI System Using Static IP Addresses Page 45 Fig. 42: Connecting the client software to a reader.3. Right-click on the de

Page 46 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader GuideFig. 43: The Instrument Properties dialog box.4. Enter the new I

Networking the ProteinChip SELDI System Using Static IP Addresses Page 47 Fig. 44: The Home tab of the ProteinChip data manager software.Edit the Read

Page 48 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader Guide2. Enter the IP address of the server into the ProteinChip data

Networking the ProteinChip SELDI System Using Static IP Addresses Page 49 3. At the command prompt, type in ‘ipconfig /all' then press Enter. 4. T

Page 50 Networking the ProteinChip SELDI SystemProteinChip SELDI System: Reader Guide

Page 51 IndexAaccessories 8–9Auto Mode 17–18enabling cassette processing 17autoloader 14Ccassettes 14connection, network 7controlling instrument 14Eel

Page 52 IndexProteinChip SELDI System: Reader Guidenetworking 25Protocol Mode 16–17Rrequirementsmounting 7power 6space 6ventilation 7Run button 16Ssaf

Life ScienceGroup06-0054 0207 Sig 1106Bulletin 10008220 US/EG Rev CBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Au

Introduction Page 1 ProteinChip SELDI System: Reader Guide Introduction The ProteinChip SELDI System, Personal Edition and Enterprise Edition, is de

Page 2 ProteinChip SELDI System: Reader Guide Mass Resolving Power Peptide Better than 1000 for [Arg 8 ]-vasopressin at 1084 Da, standard operating

Equipment Ratings Page 3 Personal Edition: 70 L/s high compression with two-stage rotary-vane backing pump Enterprise Edition: 250 L/s high compress

Page 4 ProteinChip SELDI System: Reader Guide 20–80% relative humidity, noncondensingMains supply voltage fluctuations not to exceed ±10% of the nom