Bio-rad Ligation and Transformation Module Manuel d'utilisateur télécharger pdf (Page 49)

23. Immediately place LB Amp IPTG agar plates upside down in the 37°C

incubator and incubate them overnight.

24. The next day, analyze the results, or wrap the plates in Parafilm and

place them at 4°C until required for inoculation of miniprep cultures

(see Appendix A).

Analysis of Results of Ligation and Transformation

Count the number of bacterial colonies that grew on the LB Amp IPTG

agar plates.

Note: Occasionally, satellite colonies may grow using this ligation method.

Count the large individual colonies, not the tiny colonies surrounding larger

colonies.

Transformation Number of Colonies

Ligation of gene of interest

Control plasmid

Note: If the number of colonies is very high and uncountable, enter "TNC"

for "too numerous" to count in the results table.

1 2 ... 44 45 46 47 48 49 50 51 52 53 54 ... 59 60

Pas de commentaire

Bio-rad Ligation and Transformation Module Manuel d'utilisateur Page 49