Bio-rad Ligation and Transformation Module Manuel d'utilisateur Page 12

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Optional Accessories
Vortex mixer (catalog #166-0610EDU)
Vacuum source
Agarose electrophoresis equipment
GAPDH
PCR module (catalog #166-5010EDU)
PCR Kleen™ Spin Purification module (catalog #732-6300EDU)
pGLO™ Plasmid, 20 µg (catalog #166-0405EDU)
Aurum™ Plasmid Mini Purification Module (catalog #732-6400EDU)
Electrophoresis reagents:
Small Ethidium Bromide DNA Electrophoresis Reagent Pack (catalog
#166-0451EDU)
Small Fast Blast™ DNA Electrophoresis Reagent Pack (catalog
#166-0450EDU)
Sample Loading Dye, 5x, 1 ml (catalog #166-0401EDU)
EZ Load™ 500 bp Molecular Ruler (catalog #170-8354EDU)
Sequencing and Bioinformatics module (catalog #166-5025EDU)
Refills Available Separately
Ligation module reagent refill (catalog #166-5016EDU)
Includes T4 DNA ligase, 2x ligation reaction buffer, proofreading
polymerase, pJet1.2 blunted vector, sterile water
Bgl II reagent refill (catalog #166-5018EDU)
Includes Bgl II restriction enzyme and 10x Bgl II reaction buffer
Transformation module reagent refill (catalog #166-5017EDU)
Includes transformation reagent A, transformation reagent B, 1 M IPTG,
C-growth medium
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