Bio-rad Ligation and Transformation Module Manuel d'utilisateur télécharger pdf (Page 35)

19. Retrieve the warm LP Amp IPTG

agar plate from the 37°C

incubator and pipet the entire

volume of the transformation onto

the labeled agar plate. Use an

inoculation loop to very gently

spread the bacteria around the

plate — do not spread for more

than 10 seconds.

20. Immediately place agar plate,

upside down at 37°C and

incubate overnight.

21. The next day, analyze results or

wrap plate in Parafilm and store

at 4°C until required.

37°C incubator

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Bio-rad Ligation and Transformation Module Manuel d'utilisateur Page 35