Bio-rad Ligation and Transformation Module Manuel d'utilisateur télécharger pdf (Page 18)

Chemical structure of deoxyribose sugar and deoxyribose nucleic acid (DNA).

Ligation is used to join vector DNA and insert DNA. There are two ways in

which DNA can be ligated into a cloning vector, one using DNA with

so-called sticky ends and the other using DNA with blunt ends. Unlike DNA

with blunt ends, DNA with sticky ends has one or more unpaired bases at

its ends that do not have complementary bases on the other strand of the

double helix. When a DNA fragment is generated by

Ta q

DNA polymerase

by a process like PCR, it typically has sticky ends with a single adenosine

(A). When a DNA fragment is generated by cutting a piece of DNA with a

restriction enzyme (an enzyme that cuts both strands of double-stranded

DNA), it may have either sticky ends or blunt ends, depending on the

restriction enzyme.

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Bio-rad Ligation and Transformation Module Manuel d'utilisateur Page 18