Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuel d'utilisateur télécharger pdf (Page 13)

ENrich Instruction Manual 9

3.3 Frit Replacement

The top frit may need to be replaced after extensive column use

or if increasing backpressures are noticed. Always try cleaning

the column in the reverse direction (as described in Section 3.1)

before replacing the frit. A frit kit is available which contains a frit

removal tool, 2 O-rings and 2 frits.

Figure 3 shows a column diagram to assist in the replacement of

the top frit.

Fig. 3. Column diagram.

1. Start buffer through the column at a slow ﬂow rate

(0.5 ml/min or less).

2. Remove the lower end tubing and ﬁtting from the column.

Firmly hold the bottom of the column over a sink or

container.

3. Loosen the lock nut turning it clockwise (Figure 4).

Fig. 4. Loosen the lock nut by turning it clockwise.

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Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuel d'utilisateur Page 13