Bio-rad ENrich™ High-Resolution Size Exclusion Columns Manuel d'utilisateur

ENrich™ SEC 70ENrich™ SEC 650High-Resolution Size Exclusion ColumnsInstruction ManualCatalog numbers780-1070780-1650 Please read these instructions be

6 ENrich SEC Instruction Manual Section 3: Care of the ENrich SEC Column 3.1 Regular Maintenance To maintain performance, the column should be store

ENrich SEC Instruction Manual 73.3 Column CleaningCareful preparation (especially filtration) of the sample and the buffers will maintain the colu

8 ENrich SEC Instruction Manual Fig. 2. Column diagram.1. Start a slow flow rate of buffer (0.5 ml/min or less) through the column.2. Remove the lo

ENrich SEC Instruction Manual 93.5 Storage Conditions To prevent bacterial growth in the column, it must be stored correctly. For short-term stor

10 ENrich SEC Instruction Manual Section 4: Ordering Information Catalog # Description780-1070 ENrich SEC 70 10 x 300 Column, 10 x 300 mm780-1650 EN

Life ScienceGroupSig 121210027325 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austri

Table of ContentsSection 1: Characteristics of the ENrich Size Exclusion Columns ... 11.1 Introduction ...

ENrich SEC Instruction Manual 1Section 1: Characteristics of the ENrich™ Size Exclusion Columns 1.1 IntroductionENrich prepacked columns for size

2 ENrich SEC Instruction Manual The ENrich support is compatible with aqueous solutions of 6 M guanidine-HCl and 8 M urea. Detergents and organic so

ENrich SEC Instruction Manual 3Section 2: Use of the ENrich Columns2.1 Preparation for Initial Use The column is supplied in 20% ethanol. To remo

4 ENrich SEC Instruction Manual 2.3 Sample considerations1. Always filter (0.22–0.45 μm filter) and/or centrifuge your sample to remove any particula

ENrich SEC Instruction Manual 5Figure 1. Typical chromatograms for ENrich SEC media. Detection at 280 nm. Absorbance at 280 nm may be greater dep