Bio-rad ENrich™ High-Resolution Size Exclusion Columns Manuel d'utilisateur télécharger pdf (Page 13)

ENrich SEC Instruction Manual 9

3.5 Storage Conditions

To prevent bacterial growth in the column, it must be stored

correctly. For short-term storage (up to 2 days), store it in freshly

prepared buffer. For long-term storage, ﬁrst wash the column

with 30 ml of deionized water and then with a storage solution of

20% ethanol. Use a ﬂow rate of 0.5 ml/min or less. Ensure that

the backpressure stays below the maximum operating pressure.

Always store the column with plugs at each end. Store the column

in a safe place at room temperature or 4°C. Never allow the

column to freeze.

If, during storage or shipment the column might be exposed to

temperatures above 35°C, brieﬂy (3 min under vacuum) degas the

20% ethanol storage solution before use.

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Bio-rad ENrich™ High-Resolution Size Exclusion Columns Manuel d'utilisateur Page 13