Bio-rad Chromatography Standards Manuel d'utilisateur télécharger pdf

Gel Filtration

Standard

Instruction Manual

Catalog #

151-1901

Lit102E.qxd 09/13/2002 2:35 PM Page A

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Résumé du contenu

Page 1 - Standard

Gel FiltrationStandardInstruction ManualCatalog #151-1901Lit102E.qxd 09/13/2002 2:35 PM Page A

Page 2 - Table of Contents

Table of ContentsSection 1 Introduction... 11.1 Instructions...

Page 3 - Introduction

Section 1IntroductionBio-Rad’s gel filtration standard is a lyophilized mixture of molecularweight markers ranging from 1,350 to 670,000 daltons. It i

Page 4 - 25–50 ml 0.125 ml

1.2 Recommended Volume of StandardThe general recommendation is to load no more than 1% of the column volume to avoid peak broadening effects. To achi

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3Eluent volumea. Bio-Sil SEC 125 columnEluent volumeb. Bio-Sil SEC 250 columnEluent volumec. Bio-Sil SEC 400 columnFig. 1. Separation of standard on H

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Fig. 1. a. Bio-Sil SEC 125 column (exclusion limit* ≈ 80,000).b. Bio-Sil SEC 250 column (exclusion limit ≈ 300,000).c. Bio-Sil SEC 400 column (exclusi

Page 7 - Section 2

51.3 Shelf LifeThe lyophilized standard is stable for at least 1 year when stored at 2 to 8°C. The shelf life may be extended if stored at a lower tem

Page 8 - LIT102 Rev E

Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr., Hercules, CA 94547 USA510-741-1000LIT102 Rev ELit102E.qxd 09/13/2002 2:35 PM Page 6