Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuel d'utilisateur télécharger pdf (Page 12)

8 ENrich Instruction Manual

3.2 Bed Height Adjustment

Under certain conditions of buffer composition, high ﬂow rates

or long-term use, the resin bed may compress, creating a void

between the frit and the top of the bed. Normally, the void can

be eliminated by turning the adjusting nut clockwise until the frit

just touches the top of the bed (Figure 2). If the bed compresses

at high ﬂow rates, stop the pump and loosen the top ﬁtting, then

use the adjusting nut to remove the void, retighten the top ﬁtting

and resume pumping buffer. If the bed has compressed from

long use, the top frit should be replaced as a precaution.

Fig. 2. Turning the adjusting nut raises or lowers the adaptor.

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Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuel d'utilisateur Page 12