Bio-rad ChromLab™ Software Manuel d'utilisateur télécharger pdf (Page 121)

Priming and Purging the Systems

Instrument Guide | 119

Priming and Purging the NGC Quest System

Caution: Before priming or purging the system, ensure that any attached

columns are offline. The priming process occurs at a high flow rate and can

significantly harm or destroy attached columns.

Priming and purging the NGC Quest system requires access to ChromLab. You can

perform the steps on the Chr

omLab computer or on the NGC touch screen.

Important: The instrument’s buffer lines cannot be primed when the system is

under pressure. Before opening the priming port, turn the inject valve to Waste

to release the system pressure.

To prime the system pumps

1. Immerse the inlet tubing for both system pumps A and B in a container of

buffer or HPLC grade (filtered, degassed) or other high-quality water.

2. Insert a 30 ml syringe into the priming port on system pump A.

3. Turn the priming port valve counter-clockwise one full turn to open the seal.

4. Slowly withdraw the

plunger to withdraw liquid from the port

until

air no

longer enters the syringe (approximately 10 ml)

and all tubing is filled with

liquid.

5. Turn the priming port valve clockwise to close the port.

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Bio-rad ChromLab™ Software Manuel d'utilisateur Page 121