Bio-rad ChromLab™ Software Manuel d'utilisateur télécharger pdf (Page 100)

3 | Preparing the Instrument

98 | NGC Chromatography Systems and ChromLab Software

Changing the Mixer Barrel

The mixer module is used to blend buffers from two to four separate solutions. It is

important to use a mixer with a barrel size that is based on the flow rate of

separation. Bio-Rad offers several different mixer barrel sizes for different flow rates.

The F10 systems ship with the 263

μl mixer base and top assembly. When using a

higher flow rate, the mixer volume of this base unit can be expanded by adding a

750

μl or 2 ml barrel. The F100 systems ship with 750 μl mixer base and top

assembly. The mixer volume can be increased at higher flow rates by inserting

either 2ml, 5 ml, or 12 ml barrel.

Table 10 lists the recommended barrel size for different flow rates for systems

without a buffer blending valve (gradient mode).

Table 11 on page 99 lists the recommended barrel size for different flow rates for

systems with a buffer blending valve.

Tip: For liquids that are difficult to mix, use a mixer barrel with a higher

capacity.

Table 10. Recommended NGC system mixer barrel sizes —

without buffer blending valve (gradient)

Barrel Size Flow Rate, ml/min

F10 Pump F100 Pump

263 μl

(Base and top)

0.1–1.00 n/a

750 μl

(Base and top)

1.00–9.99 1.00–4.99

2 ml 10.00–20.00 5.00–14.99

5 ml n/a 15.00–39.99

12 ml n/a 40.00–200.00

1 2 ... 95 96 97 98 99 100 101 102 103 104 105 ... 305 306

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Bio-rad ChromLab™ Software Manuel d'utilisateur Page 100