Bio-rad BioLogic QuadTec™ Detector and Components Manuel d'utilisateur Page 1

BioLogicQuadTec™UV/VIS DetectorInstruction ManualLinkLoadProgHold 0Run 1Time(min) 1(nm) 2(nm)Edit ProgClear OEvents: 2off 0 on /Signal 1

1.4.2 Power Up and Self-TestThe QuadTec detector is equipped with a universal power supply, which operates with supply voltages from90 to 260 Volts A

2.0 USING THE QUADTEC WITH THE BIOLOGIC DUOFLOW SYSTEMThe QuadTec UV/Vis detector provides a powerful addition to the analytical capabilities of the

2.1 INSTRUMENT CONTROL MODULE (ICM): FOR USE WITH THE DUOFLOW ONLYThe ICM translates the signal from the QuadTec detector and transmits it to the Bio

2.2 ELECTRICAL CONNECTIONS TO THE DUOFLOW SYSTEMThe QuadTec communicates with the BioLogic DuoFlow system via the ICM module. To connect theQuadTec t

2.3 PLUMBING CONNECTIONS TO THE DUOFLOW SYSTEMThe QuadTec has a PEEK flow cell that must be installed by the user. The detector fittings which have b

2.4 SYSTEM POWER UPTo power up the QuadTec Detector with the BioLogic DuoFlow system:1. Important: Ensure that the BioLogic DuoFlow software applicat

Figure 2-5. Manual Control Screen, Showing the QuadTec DetectorUSE WITH THE BIOLOGIC DUOFLOW SYSTEM SYSTEM INSTALLATION AND SETUP2-6BioLogic Duo-Flow

The traces shown on the Manual screen chromatogram and printed report are chosen in the ChromatogramSettings window, shown in Figure 2-6. From the sys

2.5.2 Setup Editor ScreenThe Setup screen selects devices to be included in a method. BioLogic DuoFlow software versions 3.0 andlater includes the Qua

SAFETYCaution/WarningDisconnect the QuadTec’s power cable from its power source before servicing. Refer to Chapter 5, Maintenance, for instructions on

Figure 2-10. Run Screen, Showing Chromatogram for QuadTec DetectorThe Run screen provides:• Wavelength status: The wavelengths in use by the QuadTec.

3.0 USING THE QUADTEC WITH THE BIOLOGIC HR SYSTEMThe QuadTec UV/Vis detector provides a powerful addition to the analytical capabilities of the BioLo

3.1 ELECTRICAL CONNECTIONS TO THE BIOLOGIC HR SYSTEMSet up the BioLogic HR system with the QuadTec detector on the bottom tray. Do not turn power on.

3.2 PLUMBING CONNECTIONS TO THE BIOLOGIC HR SYSTEMAfter making all the cable connections make the plumbing connections, as shown in Figure 3-3.Figure

3.3.2 Changing Operating ParametersTo change any parameter value:1. Use the green arrow keys to move the blinking cursor to the desired position on t

2. Configure SIM 1 and 2 “Units” value for the desired wavelengths (e.g., AU 280 nm and AU 260 nm.)Unit Range, Minimum = 0Unit Range, Maximum = 2Devic

AUTO CALIBRATION From Time Constant, press the green Right arrow to move to this field. Pressing thegreen Up arrow starts the procedure for the calibr

4.0 STAND-ALONE MODETurn on power to the QuadTec detector and wait while it completes its self test. If successful, the lamp willwarm to a constant w

4.2 DISPLAY AND MENU STRUCTUREAfter the QuadTec has been switched on and the self-test routine has been successfully run, the device isready for oper

AUTO CALIBRATION This procedure searches automatically for reference points within the spectra of thelamp for reliability and reproducibility of the w

ANALOG OUT Absorbance signals come from the two integrator outputs on the rear of the detector FULL SCALE and may be scaled.Full scale may be set fro

Use the Right arrow key to access the output field and select E1 or E2 asappropriate.Use the right arrow key to access the signal level. Set the minim

4.2.3 VIEW Menu The View menu gives an overview of existing programs and program links. The following figure shows anexample where programs 01, 02, 0

4.3.1 Selecting Wavelength(s)Use the green Left arrow to select the View menu and then:1. Position the cursor on one of the two wavelength fields and

4.4 PROGRAMMING THE QUADTEC UV/Vis DETECTOR4.4.1 Creating ProgramsWhen creating programs, note the following:• The QuadTec can store up to 20 progra

4.4.3 Time Programming of Absorption WavelengthsUse this instruction for programming the absorption wavelengths as a function of time.1. Access the O

4.4.4 Programming Events for Control of a Fraction CollectorThis example demonstrates a program to control a fraction collector via the QuadTec UV/Vi

4.4.5 Program ExecutionPrograms must be activated prior to execution. To execute a program:1. Enter the desired program number in the “Load Prog” fie

3. In the LINK menu, the momentary status of the running link is shown. To access this menu move thecursor to the “Load Prog” field and press the Left

TABLE OF CONTENTSTABLE OF CONTENTSSafetyChapter 1 General Description ...

The number of the scan to be performed or given out is selected by moving the cursor to the scan number,(e.g. no.:1 with the Right arrow.) The scan nu

4.6 QUADTEC DETECTOR WAKE-UP PROGRAMProgram # 30 is reserved for the Wake-Up function, enabling the lamp to be turned on or to preset awavelength pro

4.8 CONNECTING OTHER INSTRUMENTS TO THE QUADTEC DETECTOR4.8.1 Using the Event and Remote ConnectorsLocated on the rear of the QuadTec detector are t

5.0 MAINTENANCE5.1 CHECKING THE STATUS OF THE DEUTERIUM LAMPThe deuterium lamp used with the QuadTec has an extended life time to ensure long-time f

5.2 CHANGING THE LAMP1. Let the lamp cool down for at least 15 minutes after switching it off.Caution: Remove the power plug before opening the QuadT

Figure 5-2. Replacing a Halogen Lamp6. Insert the lamp’s electrical supply cable connector into the appropriate socket. The deuterium lamphas a three-

3. From the Extended Setup menu, press the Down arrow to access the desired menu items. Make thefollowing modifications:a. LAMP: The lamp type is spec

6.0 TROUBLESHOOTINGProblem Possible Cause Suggested SolutionsQuadTec detector Communicationsfaceplate does not problem between theshow up in BioLogi

Problem Possible Cause Suggested SolutionsWavelengths chosen Software options are in the Setup editor not configured detector devices box appropri

4.5 Scanning UV Spectra... 4-124.5.1 Scan Menu ...

Problem Possible Cause Suggested SolutionsExcessive pump Air bubbles trapped in pulsations exhibited the pump heads can as a sinusoidal produce exa

Problem Possible Cause Suggested SolutionsNegative peaks. The sample is applied tothe column in a differentbuffer from that used toequilibrate and elu

APPENDIX ASPECIFICATIONSLamp type Deuterium (standard)Halogen (option)User-replaceableWavelength range 190–740 nm with deuterium lamp, automatic edge

APPENDIX BWAVELENGTH SELECTION FOR TYPICAL BIOLOGICAL MOLECULESWave-length Absorbing Species Applications206 nm Carboxyl groups, Proteins, peptides,

APPENDIX CORDERING INFORMATION. SPARE PARTS AND ACCESSORIES760-1300 QuadTec DuoFlow Detector Kit; 100/110 V; 200/240 VIncludes QuadTec Detector, 3 mm

Life ScienceGroup00-0000 0000 Sig 1106Bulletin 0000 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra

1.0 GENERAL DESCRIPTIONThe BioLogic QuadTec multi-wavelength UV/Vis detector adds versatility to your biologic chromatographysystem. The QuadTec dete

1.1 USING THIS MANUALThe instructions provided in this manual are for use of the QuadTec Detector with the following systems:• The BioLogic DuoFlow c

1.3 UNPACKINGWhen you receive the QuadTec detector and system accessory kit, carefully inspect the shipping containersfor any damage, which may have

1.4 PREPARING THE QUADTEC DETECTOR FOR OPERATION1.4.1 Installation of the Flow CellThe QuadTec detector is shipped with a “dummy” flow cell installe