Bio-rad ReadySub-Cell GT Cells Manuel d'utilisateur télécharger pdf (Page 18)

Symptoms Cause Solutions

Sample wells cast Comb should be placed 1 to 2 mm

through the gel. Sample above the base of the running surface.

leaks along bottom of

running surface.

Sample overload Dilute sample.

Bands sharp but too Gel agarose percentage too high Lower agarose percentage.

few bands seen

Incomplete digestion Check enzyme activity, digest further.

High MW bands Gel agarose percentage too low Increase agarose percentage.

sharp; Low MW Switch to polyacrylamide.

bands smeared

Gels crack Too high voltage gradient, Reduce voltage. Run gel at lower

especially with low melting temperature.

temperature agarose or

low gel strength gels

Section 6

Product Information

6.1 Sub-Cell

GT Systems

Catalog

Number Product Description

170-4401 Sub-Cell GT System, with 15 x 10 cm tray

170-4402 Sub-Cell GT System, with 15 x 15 cm tray

170-4403 Sub-Cell GT System, with 15 x 20 cm tray

170-4404 Sub-Cell GT System, with 15 x 25 cm tray

170-4481 Sub-Cell GT System, with 15 x 10 cm tray and gel caster

170-4482 Sub-Cell GT System, with 15 x 15 cm tray and gel caster

170-4483 Sub-Cell GT System, with 15 x 20 cm tray and gel caster

170-4484 Sub-Cell GT System, with 15 x 25 cm tray and gel caster

170-4405 Wide Mini-Sub Cell GT System, with 15 x 7 cm tray

170-4485 Wide Mini-Sub Cell GT System, with 15 x 7 cm tray and mini-gel caster

170-4406 Mini-Sub Cell GT System, with 7 x 7 cm tray

170-4486 Mini-Sub Cell GT System, with 7 x 7 cm tray and mini-gel caster

Sub-Cell Systems with PowerPac

™

Basic Power Supply, 100—120/220—240 V

164-0302 Sub-Cell GT Cell and PowerPac Basic Power Supply

164-0301 Wide Mini-Sub Cell GT Cell and PowerPac Basic Power Supply

164-0300 Mini-Sub Cell GT Cell and PowerPac Basic Power Supply

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Bio-rad ReadySub-Cell GT Cells Manuel d'utilisateur Page 18