Bio-rad Sub-Cell® Model 192 Cell Manuel d'utilisateur

Sub-Cell®Model 96 andModel 192Agarose GelElectrophoresis SystemsInstruction ManualCatalog Numbers170-4500 through 170-4511For Technical Service Call Y

Table 2.2 Gel Volume RequirementsGel Size 0.5 cm thick 0.75 cm thick 1.0 cm thickBases25 x 10 cm (Model 96) 125 ml 185 ml 250 ml25 x 15 cm (Model 192)

base stage or UVTP tray. The following instructions describe how to manipulate the comb andcomb holder for obtaining comb height and comb holder posit

Removable Tray (UVTP) Gel CastingCasting gels on the base stage with UVTP tray1. Level the cell using the leveling bubble provided.2. Place the UVTP t

Figure 2.1 Sealing the UVTP tray for gel casting.7. Place the comb(s) into the appropriate slot(s) of the tray (refer to Section 2.2 for combadjustmen

Tape Method1. Seal the ends of the UVTP gel tray securely with strips of standard laboratory tape. Pressthe tape firmly to the edges of the gel tray t

3. Attach and tighten (10 lb.-in. torque) the straight fitting to the elbow-shaped fitting.4. Connect tubing to the elbow-shaped fittings on the safet

8. Power requirements vary depending on gel thickness, length and concentration, and typeof electrophoresis buffer used. Refer to Table 2.3 for relati

and gels appropriately (Review EtBr Material Safety Data Sheet [MSDS] for properdisposal methods).2. Destain the gel for 10-30 minutes in dH2O using t

1x Tris-Acetate-EDTA (TAE) — 40 mM Tris (pH 7.6), 20 mM Acetic Acid, and 1 mM EDTA.50x Stock (1 liter): dissolve in 600 ml distilled water:Tris Base (

3.4 DNA and RNA Sample Loading Dye1-2A convenient 10x sample buffer stock consists of 50% glycerol, 0.25% bromophenolblue, and 0.25% xylene cyanole FF

WarrantyBio-Rad Laboratories warrants the Sub-Cell Model 96 and Model 192 electrophoresis systems againstdefects in materials and workmanship for 1 ye

Do not use abrasive or highly alkaline cleaners on Sub-Cell parts. Do not expose Sub-Cellparts to temperatures >60 °C. Do not sterilize Sub-Cell pa

4.5 RNase DecontaminationSub-Cell parts can be cleaned with a mild detergent and treated for 10 minutes with 3%hydrogen peroxide (H2O2) and then rinse

Section 5TroubleshootingSymptoms Probable Causes SolutionsSlanted lanes (bands)Curved line or distortion oflanes (bands)Differential relative mobilit

Section 6Ordering Information6.1 Sub-Cell Model 96 and Model 192 SystemsCatalogNumber Product DescriptionSub-Cell Model 96 Systems170-4540 Sub-Cell Mo

CatalogNumber Product DescriptionSub-Cell Model 192 Accessories170-4515 Sub-Cell Model 192 Base170-4516 Sub-Cell Model 192 Safety Lid, with cables170-

CatalogNumber Product DescriptionSub-Cell GT Systems(continued)170-4404 Sub-Cell GT System, 15 x 25 cm tray170-4405 Wide Mini-Sub Cell GT System170-44

CatalogNumber Product DescriptionSupported Nitrocellulose Membrane (0.45 micron)161-0090 Sheets, 7 x 8.4 cm, 10161-0091 Sheets, 10 x 15 cm, 10161-0092

CatalogNumber Product DescriptionElectrophoresis Buffers161-0733 10x Tris/Boric Acid/EDTA (TBE), 1 L161-0743 50x Tris/Acetic Acid/EDTA (TAE), 1 L161-0

Section 7References1. Sambrook, Fritsch, and Maniatis, Molecular Cloning, A Laboratory Manual, Second Edition, ColdSpring Harbor Laboratory Press, 198

Australia, Bio-Rad Laboratories Pty Limited, Block Y Unit 1, Regents Park Industrial Estate, 391 Park Road, Regents Park, NSW 2143 • Phone 02-805-500

Table of ContentsPageWarranty Information ...Inside Front CoverSection 1 Gener

Section 1General Information1.1 IntroductionThe Sub-Cell instruments comprise a comprehensive and versatile gel electrophoresissystem that effectively

Before every use, inspect the base for cracks or chips. Cracks or chips may cause thebuffer to leak from the base and cause a potential electrical haz

1.3 List of System PartsEach Sub-Cell system comes with the components listed in Table 1.1. Check your instrument to insure all items are present. Not

Figure 1.1 Sub-Cell Model 96 and Model 192 Parts4Safety LidElectricalCablesRecirculation Port PlugsComb HolderCombElectrical LeadsUVTP Gel TrayComb Ho

Figure 1.2 Sub-Cell Model 96 and Model 192 Gel Caster Parts1.4 SpecificationsSub-Cell Model 96Base Footprint (L x W x H) 29 .5 cm x 29.0 cm x 9.0 cmBa

ContructionBase Cast AcrylicGel Casting Gates Anodized AluminumSafety Cover Cast AcrylicBanana Plug/Electrode Cassette Polycarbonate Banana Plugs Gold