Bio-rad C/P Lift Membranes Manuel d'utilisateur
Naviguer en ligne ou télécharger Manuel d'utilisateur pour Accessoires pour l'eau Bio-rad C/P Lift Membranes. Bio-Rad C/P Lift Membranes User Manual Manuel d'utilisatio
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C/P Lift®MembranesInstruction ManualCatalog Numbers162-0162162-0163LIT267B 10/28/98 10:12 AM Page fc1
Table of ContentsSection 1 Introduction...1Section 2 Colony Screening ...12
Section 1IntroductionC/P Lift membranes were developed for colony andplaque screening. When handling the membranes,always wear gloves or use forceps.
2.2 Replica Lifts (with amplification)1. Place a C/P Lift membrane disc on the appropriateselective agar. Plate cells containing recombinantplasmids o
11. Rinse briefly in 2 x SSC, 0.2% SDS to removecellular debris.12. Air dry 30 minutes, bake 1 hour at 80 °C.13. Hybridize (see Section 4).Section 3Pl
2. Cut one corner of the plastic bag and pipetprehybridization solution in:1 mM EDTA 0.5 M
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT267 Rev BLIT267B 10/28/98 10:12 AM Page 8
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