BIO RAD GS-900™ Calibrated Densitometer manuels

Manuels d'utilisation et guides de l'utilisateur pour Accessoires pour l'eau BIO RAD GS-900™ Calibrated Densitometer.
Nous fournissons des manuels en pdf 2 BIO RAD GS-900™ Calibrated Densitometer à télécharger gratuitement par type de document : Manuel d'utilisateur


Table des matières

2-D Electrophoresis Workflow

1

How-To Guide

1

Part II: Methods 75

3

Part IV: Appendices 125

3

Theory and

4

Product Selection

4

2-D Electrophoresis Workflow

5

Cell Lysis

8

Products for Cell Lysis and

9

Protein Extraction

9

Protein Solubilization

9

+ + HI

10

General Considerations

11

Nucleic Acids (DNA and RNA)

11

Phenolic Compounds

12

Polysaccharides

12

Subcellular Location

13

Prefractionation

13

Solubility/Hydrophobicity

14

Protein Charge

14

Products for Depletion

15

ProteoMiner Technology

16

Protein Assay Products

17

The First Dimension:

18

Isoelectric Focusing

18

ReadyStrip IPG Strips

19

Estimation of pI

20

Selection of IPG Strips

20

Sample Application

21

Setup for IEF

21

PROTEAN i12 IEF System

22

Power Conditions for IEF

22

The Second Dimension:

23

SDS-PAGE

23

Protein Separation by Size

24

Choice of Gel Size

25

Choice of Buffer System

25

7.5% 10% 12%Any kD

26

10–20%16.5%

26

10% 4–20% 8–16%

27

Detection

29

Detection of Proteins in Gels

30

Coomassie Stains

31

Fluorescent Stains

31

Protein Stains

31

Silver Stains

32

Stain-Free Technology

32

Image Acquisition

33

Analysis, and Spot Cutting

33

Imaging Systems

34

Image Analysis

34

Spot Cutting from 2-D Gels

35

Identification and

36

Characterization of

36

2-D Protein Spots

36

Beyond Excision

37

Proteolytic Digestion

37

Ion currentIon current

38

Abundance

38

Sample Preparation

39

Tips for Sample Preparation

40

Buffers and Solutions

40

Plant Leaves

42

Sample Cleanup

42

Buffer Exchange (Desalting)

42

First-Dimension IEF

44

Protocol

45

IEF with Gel-Side Down

46

Performing IEF (contd.)

46

Second-Dimension

49

Tips for SDS-PAGE

50

IPG Strip Equilibration

50

Protein Detection

52

Components:

54

In-Gel

55

Trypsin Digestion

55

Troubleshooting

57

(contd.)

58

2-D Gel Evaluation (contd.)

60

Problem Cause Solution

60

Appendices

64

Appendix A

65

Appendix B

67

Appendix C

68

TABLE OF CONTENTS

71

Life Science

72

Bio-Rad

72

Laboratories, Inc

72





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