Bio-rad Components for Older Model Spot Cutter Manuel d'utilisateur

ProteomeWorks™Spot Cutter Systemwith PDQuest Basic Excision SoftwareInstruction ManualCatalog Number165-7009For Technical Service Call Your Local Bio-

• Gel-holding barsTwo custom designed stainless steel Gel-holding Bars are included in the accessory boxwith the spot cutter. These gel holding bars a

2.8 Spot Cutter Setup• Connect the PC cable. Connect the 9-pin connector to the Com 1 serial port of the computer and the 25 pin connector to the sys

2.10 Software FeaturesTo open Basic Excision Tool:• Double click on the PDQuest icon in the desktop. • Select the menu item "Identification&quo

Recutting SpotsIf the cutting tip has not picked up a spot, click on the Recuts button below the image window.In the pop-up box, enter the numbers of

Multiple Cuts of the Same SpotTo place multiple cuts of the same spot in the same microtiter plate well, make sure theMultiple Cuts/Spot checkbox is c

Cut Run OptionsBefore beginning a cut run using the Basic Excision Tool, you must select the cutter optionsfor that run.If Spin When Cutting is select

Specify the microtiter plate size you are using by clicking on 96 wells or 384 wells. Specify the diameter of the cutting tip by selecting 1.0 mm or 1

Note: Calibration creates several parameter and image files in the Install.prm directory inthe Discovery Series directory on your hard drive. These f

3.2 Camera Calibration: FocusThe lens on the camera should be locked in place once it is focused. To accomplish this,the lens has a silver lock colla

If it is not already on, click on the Focus Cutter Camera icon in the Basic Excision Toolscreen. If the image is over exposed, check the light and ape

Safety InformationCaution SymbolRead the manual before using the ProteomeWorks Spot Cutter. For technical assistance, contact your local Bio-Rad or, i

3.4 Camera Alignment—X Crop• Place calibration template on cutting platform and click "Acquire Image" button.• Evaluate the image and deter

3.6 Calibration of the Microtiter Plate (MTP) 3.6.1 Calibration of MTP A1 PositionThe microtiter plate holder is to the immediate right of the cutti

• When the cutting tip is centered over the well, click the "Test" button to assess the clearance into the microtiter plate well. Once the t

The wells are designed to accommodate a 1.5 ml capless micro test tube, such as the Bio-Rad catalog number 223-9500.• Click "spot cutter settings

3.8 X, Y Calibration of Cutting PlatformThe interaction between the software, the computer, the camera and the spot cutter requiresan initial calibra

• Press the onscreen start key to begin the calibration process.• The Spot Cutter will proceed to cut 25 spots in an array of 5 rows with 5 spots per

Section 4Spot Excision4.1 Gel and Blot Preparation• Storage of gels can be in any type of container or plastic that keeps the gel protected andhydrat

Fig. 10. Basic Excision Tool menu selection.• Step 1: Acquire ImageThere are two default options for acquiring an image, Light ON, for gels; and Ligh

Removing a cut (or multiple cuts from the boxed area)Activate the "Remove cut" icon, place the cursor over a selected cut and click. The rem

4.4 Starting the Spot Excision Process✔ Press the “Begin” icon to start cutting the selected spots on the gel or membrane. ✔ When the spot excision r

Table of ContentsPageSection 1 Introduction...11.1 Gen

Note: The top of the cutting tip is rectangular in shape. If the wrench does not appear to fityou will need to rotate the cutting tip 90° and then th

Diagram 1 Diagram 2Diagram 3 Diagram 4✔ Ejection PinIf the ejection pin is not recovering after pushing out through the cutting tip, a small amountof

Section 6TroubleshootingCommunication ErrorIf the computer cannot make communication connection with the spot cutter or the camera, an error message w

30Things to try:1. Close PDQuest. Turn off power to the spot cutter, then turn on spot cutter and pressPause/restart button to initialize. Open PDQues

31Lights not turning on during image acquisition and cutting tip not turningduring cut• The spot cutter and computer "power on" sequence wer

In depositing spots to the microtiter plate, it is important to prepare the plates with liquid inthe wells. While deposit of the gel pieces does not d

The foregoing obligations are in lieu of all other obligations and liabilities including negligence and all warranties of merchantability, fitness for

Appendix ASystem Box (Front)1. Camera Communication Cable2. Camera 3. Camera Lens4. X-Y Movement Bar5. Start/Pause Button6. On/Off Switch7. Light Push

Appendix BWorking Surface1. Cutting Mechanism Cover2. Cutting Head Shaft Cover3. Cutting Tip4. Cutting Tip Holder5. Bubble Level6. Wash Well Station,

Appendix CSystem Box (Back)1. Main Power Plug2. Fuse for Voltage Settings3. System Communication Port4. Camera Mounting Arm5. Ground Plug3615423

Section 1Introduction1.1 General DescriptionThe ProteomeWorks Spot Cutter System is a precision instrument for the accurate location, excision and lo

GlossaryBox Refers to a dialog box. This is a box (window) whichpops up onto the screen containing dialog to be read, andthen requires a button to be

Systems RecordSerial Number Date of Purchase Purchase OrderNumber NotesService RecordDate Description of Problem Name Signature38

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2Section 2Getting Started2.1 Software DescriptionThe ProteomeWorks Spot Cutter is shipped with PDQuest Basic Excision software forselecting and then

2.2 Installing Camera Driver (PVCAM 2.5.8) and the PCI CardThe following procedure applies to new installations of PDQuest version 7.0 and higher,and

If you have one of these cases:• An older PVCAM driver needs to be uninstalled, and the new driver re-installed.• Communication error with the camera

2.4 Spot Cutter Setup• Remove the Spot Cutter instrument and accessories from the shipping container. Do notlift the instrument by the camera arm.• R

The working surface is shown in Appendix B and consists of two main areas, the cuttingsurface and the microtiter plate holder / wash wells area. In ad