Bio-rad S3™ Cell Sorter Manuel d'utilisateur Page 1

S3™ Biosafety System Class IInstruction ManualCatalog #145-1078 #145-1021 #145-1022#145-1029 #145-1030

viii | S3 Biosafety System

Instruction Manual | 1Introduction1The S3™ biosafety system is a Class I aerosol containment hood designed to work with the S3 and S3e cell sorters, w

IntroductionSystem ComponentsThe S3 biosafety system includes the following components (Figure 1): nEnclosure top assembly with vinyl side panels nHDP

Hardware OverviewHardware OverviewThe S3 biosafety system comprises three components (Figures 2 and 3): n HEPA air generator — fan system will filter a

Introduction4 | S3 Biosafety SystemThe front panel of the S3 biosafety system has two access doors (Figure 4). n Sample access door — provides acce

Instruction Manual | 5The S3™ biosafety system is a Class I system designed to protect the user and the environment from aerosols generated during the

6 | S3 Biosafety SystemOperation IMPORTANT! If the airflow through the HEPA filter falls below required limits for containment, the software will aler

Instruction Manual | 7Software ControlIf any of the following occur, the S3 or S3e cell sorter will stop sample acquisition or sort and alert the user

Operation8 | S3 Biosafety SystemWorking with the S3 and S3e Cell SortersThe S3 biosafety system was custom-designed to provide an easy access interf

Working with the S3 and S3e Cell SortersInstruction Manual | 9Fluidics Access DoorThe fluidics access door is positioned for easy access to the bulk fl

10 | S3 Biosafety SystemOperationVinyl Side Panel OperationAll four sides of the S3 biosafety system can be opened to access the S3 or S3e cell sort

Instruction Manual | 113The S3™ biosafety system has been designed to maintain the safety of the user and the outside environment. Always follow the p

12 | S3 Biosafety SystemMaintenanceFilter ReplacementReplacement of the HEPA filter requires proper training and should be performed by the certifyin

Instruction Manual | 13Whenever service is conducted on the biosafety system, be sure to completely power down both the S3™ or S3e cell sorter throug

Service and CertificationCertification ProcedureGeneral InformationThe enclosure is a ventilated cabinet for user and environmental protection. All in

Certification Procedure3. Inject PAO in such a manner to allow for uniform particle distribution across the entire face of the HEPA filter.4. Set the

Service and Certification16 | S3 Biosafety SystemS3 Biosafety System Class I Certification FormS3 Biosafety System Class I Certification Form Custome

Contaminant TestingInstruction Manual | 17Contaminant Testing This protocol is designed to test the aerosol contamination levels that occur under norm

Service and Certification18 | S3 Biosafety SystemTo start sorting the Glo Germ particles:12. Click Start on the instrument control panel to acquire

Contaminant TestingInstruction Manual | 19Sample the following locations for 10 min each: nNear sample station nFront, below bulk fluids door nOn top,

Instruction Manual | iCopyright © 2014 by Bio-Rad Laboratories, Inc. All rights reserved. Toll Free: 1-800-4-BIORAD (1-800-424-6723)Fax: 510-741-5800F

20 | S3 Biosafety System Contaminant Testing RecordCustomer Name

Instruction Manual | 21Troubleshooting5Table 7 describes possible errors and causes with troubleshooting steps. Table 7. Troubleshooting table. Error

22 | S3 Biosafety System

Ordering Information Catalog # Description 145-1078 S3 Biosafety System Class I145-1021 S3 Cell Sorter 488 1L2D with S3 Biosafety System Class I14

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Life ScienceGroup Sig 121310033232 Rev B Bio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austri

ii | S3 Biosafety SystemBio-Rad Laboratories ResourcesBio-Rad provides many resources for scientists. Table 1 lists available resources and how to l

Instruction Manual | iiiSafety and Regulatory ComplianceFor safe operation of the S3 biosafety system with use of the S3 or S3e cell sorter, we strong

iv | S3 Biosafety SystemSafe Use Specifications and ComplianceElectrical Safety Information and ClassificationThe S3 biosafety system conforms to inte

Instruction Manual | vChemical HazardsThe S3 or S3e cell sorter inside the S3 biosafety system includes a waste container that may potentially contain

vi | S3 Biosafety System

Instruction Manual | viiTable of ContentsChapter 1 Introduction ............ 1System Components ......