Bio-rad Microplate Manager Software Manuel d'utilisateur télécharger pdf (Page 55)

Analyzing Data Using Cutoffs

Reference can be made to standard or sample IDs that have been defined in the

Template setup

• Standards: enter ! followed by concentration

• Unknowns: enter identifier

• Blanks: enter #

In all Cutoff equations, Microplate Manager 6.0 uses the averaged value of that

group.

Examples of valid expressions:

• U01

• (U01+.1)/2

• (!100-!10)*2

Cutoff Examples for Assays with Controls

Positive & Negative Controls – positive (POS) & negative (NEG) control well(s)

defined on template

• Low Cutoff: NEG

• High Cutoff: POS

% B/Bo – non-specific binding blank (NSB) & maximum or Bo well(s) (MAX)

defined on template

• Low Cutoff: NSB

• High Cutoff: MAX

% Calibrator –blank (#) & calibrator (CALB) well(s) defined on template

• Low Cutoff: #

• High Cutoff: CALB

% Control - non-specific binding blank (NSB) & control well(s) (CNTRL) defined

on template

• Low Cutoff: NSB

• High Cutoff: CNTRL

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Bio-rad Microplate Manager Software Manuel d'utilisateur Page 55