Bio-rad Microplate Manager Software Manuel d'utilisateur télécharger pdf (Page 47)

Analyzing Data Using a Standard Curve

Point to Point

In the Point to Point method of analysis, the data points are simply connected. No

fitting of the data points to a line or curve is done.

For reactions where there is a lag time or a plateau, it is often convenient to look at

the data using this plot in order to see the true linear range of the assay.

Cubic Spline

Cubic spline is a piecewise polynomial approximation in which a set of data points

are joined by a series of curve fits. These multiple curve fits are smoothed by using

a cubic fit

y = Ax + Bx2 + Cx3 + D

This type of curve fit is less affected when any one of the standards has a poor value.

Because multiple curve fits are performed with this function, the fit parameters are

not shown with the graph.

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Bio-rad Microplate Manager Software Manuel d'utilisateur Page 47