Bio-rad Microplate Manager Software Manuel d'utilisateur télécharger pdf (Page 40)

Analyzing Data Using a Standard Curve

Analyzing Data

Using a Standard Curve

The standard curve is a plot of the concentration of the standards you have

designated in the template (on the x axis) versus the readings from the instrument (in

OD units for an endpoint experiment (on the y axis).

Microplate Manager 6 provides the following types of curve fit options:

• Linear

• Zero-intercept Linear

• Semi-Log

• Log-Log

• Quadratic

• Log-Logit

• 4-Parameters

• 5-Parameters

• Cubic Spline

• Point-to-Point

To perform a curve fit and display the curve fit

plot on the screen click on the Curve Fit Plot

icon and select the desired curve fit by clicking on the

Choose Fitting Function icon.

If a transformation has been applied to the template, then

the values plotted in the standard curve will be the

transformed values.

The mean value of the replicates of the standards, and not

the individual values, are used in the calculation of the

standard curve.

1 2 ... 35 36 37 38 39 40 41 42 43 44 45 ... 111 112

Pas de commentaire

Bio-rad Microplate Manager Software Manuel d'utilisateur Page 40