Bio-rad BioLogic LP System Manuel d'utilisateur télécharger pdf (Page 19)

Fig. 7.

Section 22

Final Check Before Starting Method

1. Press the MANUAL soft key, then pres the START soft key to start the

pump.

2. Make sure that “A” buffer is selected.

3. Check the plumbing connections for leaks, check for air in the lines

and purge if necessary.

4. Press the instrument keys to check settings.

a. UV monitor: Range 0.05 AUFS. Rezero the UV monitor if

necessary. (Only zero the monitor if fluid is moving through the

flow cell.)

b. Conductivity: Minimum and maximum values match the values for

“A” and “B” buffers that were determined when purging the system.

c. Method: Press Program, then View Method — Review gradient

and fraction collector program.

d. LP Data View software: Open the LP Data View software and

press the Record button. Zero the UV monitor on the BioLogic LP

workstation, making sure that the UV trace flatlines at zero. Press

the Stop button, then the Clear button. Do not save this trace. The

software is now ready for the sample run to begin.

BioLogic LP

or Econo

Gradient

Pump

Column

MV-6

Inject

Valve

Sample Loop

Waste

Inject

Port

FILL

"FILL" POSITION

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Bio-rad BioLogic LP System Manuel d'utilisateur Page 19