Bio-rad Bio-Scale™ CHT™ Type I Columns Manuel d'utilisateur Page 1

Bio-Scale CeramicHydroxyapatite,Type I ColumnsInstruction ManualCatalog Numbers751-0021, 751-0023,751-0025, 751-0027For Technical Service Call Your Lo

3.6 References1. Guide to Sample Preparation. Bio-Rad Laboratories Technical Bulletin # 1322.2. Hydroxylapatite. T. L. Brooks (Calbiochem Biochemicals

Bio-Rad LaboratoriesAustralia, Bio-Rad Laboratories Pty Limited, Block Y Unit 1, Regents Park Industrial Estate, 391 Park Road, Regents Park, NSW 214

Table of ContentsPageSection 1 Characteristics of the Bio-Scale CHT2, CHT5, CHT10, CHT20 Ceramic Hydroxyapatite, Type I Columns...

Section 1Characteristics of the Bio-Scale CHT2, CHT5,CHT10, CHT20 Ceramic Hydroxyapatite, Type IColumns1.1 IntroductionBio-Scale prepacked hydroxyapat

2Table 1. Column Characteristics CHT2-ICHT2-I CHT5-I CHT10-I CHT20-IColumn volume (ml) 2 5 10 20Recommended max.protein loading (mg) 20 50 100 200Reco

or diluted to the start buffer’s concentration. Buffer exchange can be accomplished usingBio-Spin®6 or Bio-Spin 30 columns, Econo-Pac®10DG desalting c

Fig. 1. Schematic gradient for separation on Bio-Scale CHT2-I column.2.4 Use of DetergentsMost of the common detergents used for protein isolation may

sharp, UV-absorbing peaks during the gradient, complicating subsequent analysis of the chro-matogram.Section 3Care of the Bio-Scale CHT-I Columns3.1 C

3.4 Top-Off ResinIf the top of the resin bed becomes fouled and the above hygiene steps do not restore per-formance, then a few mm of the bed should b

Fig. 2. Instructions for removal of top frit.1. Remove the upper and lower end fittings from the column.2. Remove the upper lock nut from the top end