Section 1
Introduction
With the Mini-PROTEAN II multiscreen apparatus you can quickly and easily screen up to
40 different antibody or serum samples on Western blots, without having to cut the membranes into
strips. Only 600 µl of sample is used per channel, eliminating waste of precious antibody. Two indi-
vidual and independent sample templates allow screening of either one or two mini blots.
The multiscreen apparatus is simple to operate. Electrophorese an antigen sample on an SDS-
PAGE mini gel using the Mini-PROTEAN II cell, and blot it onto nitrocellulose or Zeta-Probe
®
membrane with the Mini Trans-Blot
®
, Trans-Blot, or Trans-Blot SD cell. After blocking the unre-
acted sites, clamp the membrane between the gasket and sample template. The assembly is held
together with four screws, and the rubber sealing gasket prevents any well-to-well leakage. Pipet
serum or antibody samples into each of the channels for incubation with the anitgen. Wash solu-
tions can be easily introduced with the Eppendorf
®
Repeater
™
pipet, and are rapidly removed by
vacuum aspiration. The Multiscreen apparatus is compatible with all common Western blotting
procedures.
1.1 Specifications
Materials
Multiscreen apparatus Acrylic plastic
Multiscreen gasket Silicone rubber
Shipping weight 1.4 kg
Overall size 11 x 27 x 6 cm (W x L x H)
Membrane size 7 x 8.4 cm
Channel dimensions 2.5 mm x 5.2 cm x 5 mm (W x L x H)
Channels per sample template 20
Section 2
Equipment and Reagents
Catalog Number Product Description
170-4017 Mini-PROTEAN II Multiscreen Apparatus, includes Multiscreen
sample templates, 2 Gaskets and Base Plate
170-4018 Mini-PROTEAN II Multiscreen Gaskets, 2
Mini-Protean II Cell
165-2940 Mini-PROTEAN II Cell, includes 10 well combs, 0.75 mm
spacers (4), Electrode Core with Gaskets, lower buffer
chamber, lid with cables, 3 sets Glass Plates, Clamp
Assemblies (2), Casting Stand with Gaskets, leveling bubble,
and instructions
Transfer Cells
170-3930 Mini Trans-Blot Electrophoretic Transfer Cell
170-3935 Mini Trans-Blot Module
170-3946 Trans-Blot Electrophoretic Transfer Cell, with plate electrodes
170-3910 Trans-Blot Electrophoretic Transfer Cell, with standard electrodes
170-3940 Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
1
(10 pages)
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