Bio-rad Model 225 Tube Gel Casting Stand Manuel d'utilisateur

Mini-PROTEAN®2-DElectrophoresis Cell,Mini-PROTEAN Tube Cell, andMini-PROTEAN Tube ModuleInstructionManualCatalog Numbers165-2960,165-2961 and 165-2965

ImportantThis Bio-Rad instrument is designed and certified to meet IEC1010-1* safety standards. Certified products are safe to use when operated in ac

4. After adding the catalysts, fill a syringe with monomer solution, and attach aneedle.Caution: Wear gloves when handling acrylamide!5. Insert the ne

Section 6Running the First-Dimension (IEF)6.1 Electrode Preparation1. Insert a gel tube with reservoir or a stopper into each of the 16 positions in

6.2 Sample PreparationAdd an equal volume of first-dimension sample buffer to the sample. Incubate atroom temperature for 10–15 minutes. The sample r

Section 7The Second-DimensionWhile the first-dimension tube gels are running, cast the second dimensionslab gels according to the directions in the Mi

7. To equilibrate the tube gel, overlay it with enough SDS sample buffer to cover itcompletely, (about 200 µl), and allow it to sit for up to 10 minut

2. First-dimension gels do not focus a. Check the tube gels, tubing connectors,well. sample reservoir and ends of the gelsfor bubbles.b. Check the pH

c. Vertical streaking that is connected to aprotein spot may be caused by proteinaggregation, incomplete reduction, orincomplete alkylation in the sam

AppendixElectrophoresis ChemicalsCatalog Quantity/Number Product Description Package161-0100 Acrylamide, 99.9% 100 g161-0101 Acrylamide, 99.9% 500 g16

Catalog Quantity/Number Product Description Package161-0407 Triton X-100 Detergent 500 ml161-0730 Urea 250 g161-0731 Urea 1 kg161-0460 CHAPS 1 g161-04

Table of ContentsPageSection 1 Introduction...1Section 2 Equipmen

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NoteTo insure best performance from the Mini-PROTEAN 2-D electrophoresis cell, becomefully acquainted with these operating instructions before using t

Section 1IntroductionThe benefits of performing 2-D electrophoresis in a mini-format lie primarily inthe reduced time required to complete the entire

2Mini-PROTEAN tube cell (catalog # 165-2961). Includes tube cell module (1), sample reservoirs (2),capillary tube connectors (3), tube setup; sample

2.3 Additional Required EquipmentThe following additional material is required:1. 1 ml and 10 ml syringes2. Sample loading syringes, use a 20–100 µl

Section 3Solutions3.1 Solutions for First-Dimension1. First-dimension acrylamide stock solutionAcrylamide/bis (30% T/5.4% C)Acrylamide 28.38 gBis-acr

3.2 Preparation of First-Dimension Tube Gels1. First-dimension gel monomer solution8.0 M urea 5.5 g4% acrylamide (total monomer) 1.33 ml acrylamide s

Section 4Safety InstructionsPower to the Mini-PROTEAN tube cell is supplied by an external DC voltagepower supply. This power supply must be ground is