Bio-rad Mini-PROTEAN® 3 Dodeca™ Cell Manuel d'utilisateur

Mini-PROTEAN®3 Dodeca™CellInstruction ManualCatalog Number165-4100For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIOR

Problem Cause Solutionb. Ionic strength of sample b. Use same buffer in lower than that of gel sample as in gel orstacking gel.8. Skewed or distorted

CatalogNumber DescriptionMini-PROTEAN 3 Multi-Casting Chamber165-4110 Mini-PROTEAN 3 multi-casting chamber*, includes acrylic blocks andseparation she

CatalogNumber DescriptionModel 485 Gradient Former165-4120 Model 485 Gradient Former, includes body with valve stem and tubing con-nection kitPower Su

Section 6Warranty InformationThe Mini-PROTEAN 3 Dodeca cell is warranted for 1 year against defects in materialsand workmanship. If any defects should

4006191 Rev C

Table of ContentsPageSection 1 General Information ...11.1 Introduction.

Section 1General Information1.1 IntroductionThe Mini-PROTEAN 3 Dodeca Cell is a multi-cell for mini-vertical gel electrophoresis.It can run four to tw

2Important: This Bio-Rad product is designed and certified to meet *IEC61010-1 andEN61010-1 safety standards. Certified products are safe to use when

Section 2Setting up the Dodeca Cell for Electrophoresis2.1 Buffer preparationPrepare 3.4–4.4 liters of running buffer (minimum/maximum) for electroph

2.3 Assemblya. Set the clamping frame to the open position.b. Place gel sandwich or gel cassette into the clamping frame with the Mini-PROTEAN 3short

d. While gently squeezing the gel sandwiches or cassettes against the green gaskets, slide thearms of the clamping frame over the gels, locking them i

2.4 Required Accessories for CoolingThe Mini-PROTEAN 3 Dodeca cell has a cooling coil with quick connect fittings thatmust be connected to a refriger

Section 4Troubleshooting GuideProblem Cause Solution1. Smile effect – band pattern a. Center of the gel running a. Improper cooling.curves upward at b