Bio-rad 2-D Electrophoresis Workflow Manuel d'utilisateur

Oriole™ Fluorescent Gel StainInstruction Manual Catalog # 161-0495, 1x solution, 200 ml161-0496, 1x solution, 1 L161-0497, kit for 5 L For technical

6Section 2Instructions2.1. General ConsiderationsBest results are obtained by using clean technique. Any dust or dirt transferred to the surface of th

7same general order as silver stain or other fluorescent protein stains. Oriole fluorescent gel stain has a wide dynamic range, and variability in the a

8Molecular weight standards that have been prestained with a visible dye such as Precision Plus Protein™ All Blue, Dual Color or Kaleidoscope™ prestai

9NOTE: Use only methanol in preparing staining solution from the 5 L kit. The use of water, ethanol, or other solvents will result in poor staining pe

102. Stain for 90 min for maximum sensitivity. 3. Cover the tray to exclude light if the gel is stained longer than 90 min.NOTE: Best results are

112.4. Gel ImagingGels stained with Oriole stain are visualized using UV light excitation. Bio-Rad Molecular Imager Gel Doc XR+, Molecular Imager Che

12Manufacturer Imaging system Recommended settingsBio-Rad Gel Doc, ChemiDoc, For Gel Doc and ChemiDoc Laboratories VersaDoc, EXQuest system

13Manufacturer Imaging system Recommended settingsUVP BioDoc-It, Midrange (302 nm) VisiDoc-It, UV excitation with ethidium DigiDoc-It, bro

14Section 3Technical Information3.1. Sensitivity of Staining — 1-D GelsThe dye in Oriole™ stain is highly fluorescent and binds tightly to proteins. Ba

153.2. Compatibility With Mass SpectrometryOriole fluorescent protein gel stain is fully compatible with downstream proteolysis and mass spectrometric

Table of ContentsSection 1: Introduction and General Information 11.1 Introduction 11.2 Product Description 21.3 Storage 21.4 Materials and Eq

16Fig. 4. Linearity of Oriole stain. A dilution series of carbonic anhydrase was run on a Criterion gel, stained with Oriole stain, and imaged on the

173.5. 2-D Gel StainingOriole fluorescent gel stain is ideal for staining 2-D polyacrylamide gels. Clear background-free images are obtained without in

18Section 4TroubleshootingcontinuedProblem Possible Cause RemedyBands or spots No protein on gel Verify that there is actually not visible

19Problem Possible Cause RemedyPoor staining Reuse of the stain Reuse of Oriole stain is sensitivity not recommended. Use of Use only metha

20Problem Possible Cause RemedyHigh or uneven Reagent impurities Use high quality reagents. background when preparing stain staining soluti

21Section 5Product Information5.1. Oriole Fluorescent Gel StainCatalog # Description161-0495 Oriole™ Fluorescent Gel Stain, 1x solution, 200 ml 16

22Bio-Rad Laboratories, Inc. is licensed by Invitrogen Corporation to sell SYPRO products for research use only, under U.S. patent 5,616,502.AlphaImag

Life Science Group 10-0361 0510 Sig 110910017295 Rev B US/EGBio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australi

Section 3: Technical Information 143.1 Sensitivity of Staining — 1-D Gels 14 3.2 Compatibility with Mass Spectrometry 153.3 Protein-to-Protein V

1Section 1Introduction and General Information1.1. Introduction Oriole™ fluorescent gel stain is an easy to use, rapid, and sensitive stain for visuali

21.2. Product DescriptionOriole fluorescent gel stain comes in three package configurations. The 200 ml size — fully diluted and ready to use; provides

3before using. Avoid prolonged exposure to temperatures greater than 37°C and protect from light.1.4. Materials and Equipment Required but Not Suppli

41.6. Safety ConsiderationsOriole fluorescent gel stain is a dilute solution of a fluorescent dye. The working solution is flammable and should be handle

5Fluorescence, normalized120100806040200200 300 400 500 600 700 800Wavelength, nmFig. 1. Fluorescence excitation and emission spectra of Oriole