Bio-rad Criterion™ Blotter Manuel d'utilisateur

Criterion™BlotterInstruction ManualCatalog Numbers170-4070170-4071For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIOR

2. Current limits The PowerPac 200 Power Supply is capable of a 200 watt output. Unless a current limitis set, uncontrolled conductivity changes may r

10. These variables will change total resistance and thus the current readings:• Alterations in buffer make-up, i.e., addition of SDS, or changes in i

CAPS Buffer with 20 % Methanol10 mM CAPS (3-(cyclohexylamino)-1-propane sulfonic acid), 20% v/v methanol, pH 11From dry reagents:Mix 2.21g CAPS in 600

Section 4Strategies for Optimizing Electro-Elution4.1 Optimizing Protein TransferGenerally, quantitative elution of denatured high molecular weight p

5. Alter membrane type. As mentioned in 4e, PVDF membrane allows transfer in reducedalcohol(see Section 5.1). PVDF can increase the binding of low mol

pore sizes in the gel. Elimination of alcohol from SDS-protein transfers results in considerably diminished binding. Adding SDS (up to 0.1%) to the tr

Section 6Troubleshooting Guide6.1 Electrophoretic TransferPoor or no electrophoretic transfer (as detected by staining the gel)1. Transfer apparatus

2. The membrane is not properly wet or has dried out.• White spots on the nitrocellulose membrane indicate dry areas where protein willnot bind. If we

2. The membrane may have been allowed to dry during handling.• A completely wet membrane has a gray, translucent appearance. White spots willform on t

CatalogNumber Product Description170-4072 Criterion Blotter–170-4070 (with Plate electrodes) and PowerPac200 Power Supply, 110/120 V170-4073 Criterion

Table of ContentsPageSection 1 General Information...11.1 Introducti

14. Holland, L. J. and Wangh, L. H., Nuc Acids Res., 10, 3283 (1983).15. Syminton, J., Green, M. and Brackmann, K., Proc. Nat. Acad. Sci., 78, 177 (19

Section 10WarrantyThe Criterion Blotter electrophoretic transfer cell is warranted for one (1) year againstdefects in materials and workmanship. If an

Life ScienceGroupWeb sitewww.bio-rad.com Bio-Rad Laboratories Main OfficeAlso in: AustraliaPh. 02 9914 2800, Fx. 02 9914 2889 AustriaBelgium Ph. 09

Section 1General information1.1 IntroductionThe Criterion Blotter is an electrophoretic transfer cell designed for use with Criterionprecast gels as

21.2 SpecificationsCriterion Blotter tankOverall dimensions 11.8 x 21.8 x 15 cmMaterial Molded polysulfoneBuffer requirement 1.3 litersElectrodesElec

Bio-Rad is not responsible for any injury or damage caused by the use of this instrumentfor purposes other than for which it is intended or by modific

4. Set up the gel/membrane sandwich:a. Pour chilled transfer buffer into each compartment of the gel/blot assembly tray.b. Place the membrane (nitroce

h. Place a piece of filter paper on top of the membrane. Run the roller gently over thetop of the filter paper to remove any air bubbles trapped in th

2.3 Acidic TransfersIf transferring under acidic buffer pH, switch the gel and membrane in the set up instructions or simply reverse the orientation

Table 3.3 Native GelsThese conditions were determined empirically using 12.5% Tris-HCl Criterion gels and fournative protein samples: cytochrome C (