Bio-rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Manuel d'utilisateur télécharger pdf

This guide can be used to prepare and run a full 1 x 96-well assay plate.

Refer to the complete instruction manual for more information on a given

step. New users can download the manual, which includes detailed

instructions and a list of kit components, at www.bio-rad.com/bio-plex.

Initial Preparation

1. Plan the plate layout.

2. Start up/warm up the Bio-Plex

system (30 min).

Bring assay buffer, wash buffer, and sample diluent to room

temperature (RT). Keep other items on ice until needed

Begin to thaw frozen samples

3. Prime wash station for ﬂat bottom plate or set vacuum manifold to

–1 to –3" Hg for ﬁlter plate.

4. Calibrate the Bio-Plex system by following the prompts within

Bio-Plex Manager

™

software. This can be done now or during an

assay incubation step.

5. Reconstitute a single vial of standards in 500 µl of a diluent similar to the

ﬁnal sample type or matrix. Vortex for 5 sec and incubate on ice for 30 min.

Bio-Plex Pro

™

Assays

Quick Guide 4

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.

Deviation from the protocol may result in low assay signal and assay variability.

For use with Instruction Manual #

Human, Mouse, and Rat Cytokine Assays 10014905

Sample Type Diluent for Standards Add BSA

Serum and plasma Standard diluent None

Culture media, with serum Culture media None

Culture media, serum-free Culture media To 0.5% ﬁnal (w/v)

1 2 3 4

Résumé du contenu

Page 1 - Bio-Plex Pro

This guide can be used to prepare and run a full 1 x 96-well assay plate. Refer to the complete instruction manual for more information on a given

Page 2

6. Prepare a fourfold standard dilution series and blank as shown below. Vortex for 5 sec between liquid transfers. If mixing diabetes assays with

Page 3 - Running the Assay

Running the Assay 1. Prewet ﬁlter plate with 100 µl Bio-Plex assay buffer (skip for ﬂat bottom). 2. Vortex the diluted (1x) beads for 10–20 sec.

Page 4 - Laboratories, Inc

11. Wash the plate three times with 100 µl wash buffer. 12. Vortex the diluted (1x) SA-PE. Add 50 µl to each well.13. Repeat Step 5. See table for in

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Bio-rad Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Manuel d'utilisateur

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