Bio-rad Bio-Plex® Assay Builder Manuel d'utilisateur

Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr.Hercules, CA 94547 USA1-800-424-6723 (in the US)4110018 Rev C 0406Instruction ManualFor technical

Section 7Data AcquisitionRecommendations for acquiring data using the Bio-Plex suspension arraysystem are listed below. Alternatively, refer to the Bi

5. Then select the Plate Groupings tab to display the plate grouping tools.a) Select Group and drag the cursor across all the wells to define

6. If acquiring data from more than one plate, empty the waste bottleand refill the sheath bottle after each plate. Select Wash BetweenPlates

21Expired beads, detection antibody,and/or streptavidin-PE usedIncorrect incubation temperature used during incubation stepsIncubation time insufficie

Section 9Safety ConsiderationsEye protection and gloves are recommended while using this product.Consult the MSDS for additional information.Human Sou

Section 10ReferencesFulton R, McDade R, Smith P, Kienker L, and Kettman J Jr, Advancedmultiplexed analysis with the FlowMetrix system, Clin Chem 43,17

Table of ContentsSection 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . 1Section 2 Principle . . . . . . . . . . . . . . . . . .

Section 1IntroductionBio-Plex phosphoprotein assays and Bio-Plex total target assays arebead-based multiplex assays (Luminex xMAP technology) that det

Section 3Required and RecommendedMaterialsRequired MaterialsThe following are required for phosphoprotein detection: 1) Bio-Plexphosphoprotein or tota

Section 4StorageStore the individual components as specified. Note that lysates areshipped and stored separately from the coupled beads and detectiona

3. Prepare an adequate volume of lysing solution (refer to the table onthe left). For 10 ml of lysing solution, add 40 µl of factor 1and 20 µl of fact

Section 6Assay InstructionsThe following instructions apply to Bio-Plex phosphoprotein and totaltarget singleplex, custom-premixed, and mutliplex assa

Prepare Coupled Beads Protect the beads from light by covering the tubes with aluminum foil.Keep all tubes on ice until ready to use. Coupled beads mu

Assay ProcedureBring all buffers to room temperature. Avoid bubbles when pipetting.1.Wash the desired number of wells in a 96-well filter plate. If fe