Bio-rad Human Metabolic and Hormone Assays Manuel d'utilisateur télécharger pdf

This guide can be used to prepare and run a full 1 x 96-well assay plate.

For more information on a given step, refer to the corresponding section

of the complete instruction manual. New users can download the manual,

which includes detailed instructions and a list of kit components, at

www.bio-rad.com/bio-plex.

A. Reagent Preparation

1. Reconstitute the following lyophilized reagents in dH

0 before use,

according to the table below.

Reagent Volume, µl Reagent Volume, ml

Standards Mix 150 Blocking Buffer 1.5

Control 1 100 Standard Diluent 1.0

Control 2 100 Detection antibiotics 4.8

a. Allow vial to sit at room temperature for a minimum of 5 min, not to

exceed 30 min.

b. Mix by vortexing at a medium setting.

2. Bring the 10x assay buffer to ambient/room temperature (RT).

a. Mix by inversion to ensure all salts are in solution.

b. Prepare 1x assay buffer — dilute 1 part 10x assay buffer (60 ml) with

9 parts of dH

0 (540 ml).

B. Dilution of Standard (1:3 Serial Dilution)

1. Label 9 polypropylene tubes S1 through S8 and Blank.

2. Transfer the reconstituted standard into the tube labeled S1.

Bio-Plex Pro

™

RBM Metabolic and

Hormone Assays

Quick Guide

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.

Deviation from the protocol may result in low assay signal and assay variability.

9. Add 20 µl of diluted SA-PE to the required plate wells.

SA-PE Dilution Volume of SA-PE, µl Volume of 1x Assay

Buffer, µl

Total Volume, µl

1:10 225 2,025 2,250

10. Cover and incubate at 850

50 rpm, as in step 4, for 30 min at R T.

11. Wash the plate three times with 100 µl 1x assay buffer.

12. After the ﬁnal wash, resuspend the beads in 100 µl 1x assay buffer. Cover

plate, as in step 4, and shake the plate at 850

50 rpm for 30 sec.

13. Remove the plate seal and read plate at low PMT (Bio-Plex

200),

standard PMT (Bio-Plex 3D), or default settings (Bio-Plex

MAGPIX

™

Bio-Plex Pro RBM Metabolic and Hormone Assays Quick Guide

For Use with Instruction Manual #

Bio-Plex Pro

™

RBM Metabolic and Hormone Assays 10041818

The Bio-Plex suspension array system includes ﬂuorescently labeled microspheres and

instrumentation licensed to Bio-Rad Laboratories, Inc. by Luminex Corporation. MAGPIX is a

trademark of Luminex Corporation.

Bio-Plex Pro RBM kits are manufactured by Myriad RBM. Myriad RBM is a trademark of Myriad

RBM, Inc.

Life Science

Group

Sig 121310041817 Rev B US/EG

Bio-Rad

Laboratories, Inc.

Web site www.bio-rad.com USA 800 424 6723

Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11

Brazil 55 11 3065 7550 Canada 905 364 3435 China 86 21 6169 8500

Czech Republic 420 241 430 532 Denmark 44 52 10 00

Finland 09 804 22 00 France 01 47 95 69 65 Germany 089 31 884 0

Greece 30 210 9532 220 Hong Kong 852 2789 3300

Hungary 36 1 459 6100 India 91 124 4029300 Israel 03 963 6050

Italy 39 02 216091 Japan 81 3 6361 7000 Korea 82 2 3473 4460

Mexico 52 555 488 7670 The Netherlands 0318 540666

New Zealand 64 9 415 2280 Norway 23 38 41 30

Poland 48 22 331 99 99 Portugal 351 21 472 7700

Russia 7 495 721 14 04 Singapore 65 6415 3188

South Africa 27 861 246 723 Spain 34 91 590 5200

Sweden 08 555 12700 Switzerland 026 674 55 05

Taiwan 886 2 2578 7189 Thailand 1800 88 22 88

United Kingdom 020 8328 2000

1 2 3 4

Résumé du contenu

Page 1 - Hormone Assays

This guide can be used to prepare and run a full 1 x 96-well assay plate. For more information on a given step, refer to the corresponding section

Page 2 - D. Dispensing of Reagents

3. Add the appropriate amount of the standard diluent into the labeled tubes according to the table below (this will be sufﬁcient for duplicate st

Page 3

3. Add the appropriate amount of the standard diluent into the labeled tubes according to the table below (this will be sufﬁcient for duplicate st

Page 4

This guide can be used to prepare and run a full 1 x 96-well assay plate. For more information on a given step, refer to the corresponding section

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