Bio-rad Quantum Prep Plasmid Kits Manuel d'utilisateur Page 1
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Quantum Prep™Plasmid Midiprep KitInstruction ManualCatalog Number732-6120For Technical ServiceCall Your Local Bio-Rad Office orin the U.S. Call 1-800-
Table of ContentsSection 1 Introduction ...11.1 Overview...
Section 1Introduction1.1 OverviewThe Quantum Prep plasmid midiprep kit has beenoptimized for the rapid purification of DNA from 40 to 50 ml of liquid
plasmids, as that will not usually result in higher yields usingthis protocol. For low-copy number plasmids (e.g. pBR322),up to 60 ml of culture can b
5. Add 5 ml of Neutralization Solution. Cap the tube and mixby inverting the tube 6–8 times. The solution shouldbecome cloudy and develop a flocculant
Section 3References1. U.S. Patent 5,075,430 issued to Bio-Rad Laboratories.2. Ausubel et al., Current Protocols in Molecular Biology,Wiley-Interscienc
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