Bio-rad Quantum Prep Plasmid Kits Manuel d'utilisateur télécharger pdf (Page 11)

2.3 Helpful Hints

1. It is recommended that the cells do not sit longer than five

minutes at step 3 (lysis) before proceeding to step 4.

Additionally, it is recommended that the cells do not sit longer

than ten minutes at step 4 (neutralization) before proceeding to

step 5.

2. Due to the fixed angle for most microcentrifuges, the matrix may

pellet against the side of the spin filter. For optimal elution of

DNA from the matrix in step 10 of the protocol, the elution

liquid (water or TE) should be applied at the highest portion of

the matrix near the side of the spin column.

3. The use of strains deficient in the endonuclease I gene product,

such as DH5αF', is recommended for improving the quality of

plasmid DNA prepared from minipreps.

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Bio-rad Quantum Prep Plasmid Kits Manuel d'utilisateur Page 11