Bio-rad UNOsphere™ Rapid S Media Manuel d'utilisateur

UNOsphere™rapid SCation Exchange Media Instruction ManualCatalog #s156-0211156-0213156-0215156-0217Please read these instructions prior to using UNOsp

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Table of ContentsSection 1 Introduction ...1Section 2 Technical Description ...

1Section 1IntroductionUNOsphere rapid S ion exchange media is a hydrophilic spherical polymeric beaddesigned for the separation of proteins, nucleic a

2Section 3PreparationUNOsphere rapid S media is supplied fully hydrated in 20% ethanol as a 50% (v/v)slurry. For column packing, removal of the shippi

3Packing Large ColumnsIn large columns, UNOsphere rapid S should be packed using a 20–50% slurry,ideally using a combination of axial and flow compres

4Fig. 1. A simulated chromatography profile from which HETP and Asvalues arecalculated. a b 10% of peak hihWh, width at half heigBed height = 20

5Section 6Operation and MaintenanceUNOsphere media were designed to achieve the highest productivity (grams ofdrug per operational hour per liter of m

6Table 2. Common Buffers for Cation Exchange Chromatography. Buffer Buffering RangeAcetic acid 4.8–5.2Citric acid 4.2–5.2HEPES 6.8–8.2Lactic acid 3.6

7 Section 10Regulatory SupportRegulatory support files are available for UNOsphere rapid S media. If you needassistance validating the use of UNOspher