Bio-rad Bio-Rex® 70 Cation Exchange Resins Manuel d'utilisateur

Bio-Rex®Weakly AcidicCation Exchange ResinInstruction ManualCatalog Numbers142-5822, 142-5832,142-5842, 142-5852,143-5832, 143-5852,LIT206B 6/17/98 3

Section 8Product InformationMinimumWet DensityCatolog Dry Mesh Ionic Pkg. Capacity Diameter (Nominal)Number Designation Form Size (meq/ml) (microns) g

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT-206 Rev BLIT206B 6/17/98 3:07 PM Page BC1

Table of ContentsSection 1 Introduction ...1Section 2 Technical Description...1Section

Section 1IntroductionBio-Rex 70 resin is a weakly acidic cationexchanger. This resin is used for the purification andfractionation of peptides, protei

Table 1. Properties of Bio-Rex 70 ResinWet density 0.6 ml/g (Na+and H+forms)% moisture 65-74% (Na+form); 55-65%(H+ form)Swelling1 ml (H+form); 1.7 ml

size and narrower size distribution ranges. Particle size isgiven either in mesh size or micron size. Mesh refers tothe number of openings per inch on

the pH and conductivity of the effluent are the sameas that of the influent, the resin is fully equilibratedand ready for sample application.Section 4

with 5 grams of resin for 100 ml of sample, and thenoptimize the volumes after obtaining the results.2. Insure that the resin is in the proper ionic f

Section 6Resin RegenerationBio-Rex 70 resin is most efficiently regenerated in acolumn. Regenerate to the sodium form by washing with3 bed volumes of

Table 4. (Continued)Purification of inhibitory pro- Fearon, D. T., Proc. Nat. Acad. Sci. USA, 76, tein of human erythrocytes 5867 (1979).Measurement o