Bio-rad Bio-Plex® 200 Systems Manuel d'utilisateur

2.2 Description of System ComponentsThe Bio-Plex 200 system is comprised of the following components:• Array reader — combines 2 lasers, fluidics, an

Table 1. Array Reader Front and Side Panel Features.Feature DescriptionSample arm The sample arm transports the sample from the 96-wellmicrotiter pl

Table 2. Array Reader Rear Panel Features.Feature DescriptionCommunications The DB9-PIN connector is used to connect the array port P1 reader to the

2.2.2 Microplate PlatformThe microplate platform (Figures 4 and 5) allows the automated processing of samples from a96-well microplate. The features

2.2.3 High-Throughput Fluidics (HTF) The Bio-Plex HTF (high-throughput fluidics) is designed to automate the introduction of sheathfluid into the arr

2.2.4 Computer and MonitorThe Bio-Plex 200 system may be supplied with a computer. If so, please transfer the computer’sregistration to your company’

2.3 Recommended Additional Equipment Not ProvidedSurge protectorWe recommend the use of a 6-outlet surge protector, with a minimum surge current of 1

4. The maximum distance between the computer and the microplate platform and array readershould be 1.5 m (5 ft), the length of the communications cabl

• Communication cable• Sheath fluid bottle• Sheath fluid waste bottle• 1 sample needle (11.6 cm/4.6 in)1. Unpack all components, being careful not to

Fig. 12. Completed connections of computer and monitor to the Bio-Plex suspension array system.3.5 Connecting the Sheath Fluid and Waste Containers1

Fig. 13. Waste and sheath fluid bottle connections.Note: To set up the 20 L sheath fluid cube container for use, remove tape from box and lift offthe

162. Insure that the array reader, microplate platform, and HTF unit are turned off.3. Insert the Bio-Plex Manager CD-ROM into the CD drive of the com

Fig. 15. Sample needle assembly.4. Remove the sample needle by grasping the needle and gently pushing up.5. Insert the new needle into the sample arm

Fig. 16. Sample needle dialog.5. Select the plate type being used.6. Click Eject/Retract to eject the plate holder.7. Place the MCV plate on the micr

Fig. 17. Sample needle assembly.12. By holding onto the needle height adjustment thumbscrew on the needle arm, manuallymove the needle so that it jus

2. Click the Eject icon. Insert the MCV plate into the microplate platform. Click Retract.3. Choose Instrument from the main menu bar. Choose Addition

Fig. 19. Device status window.6. After the pressure has stabilized, record the air and sheath pressure (Figure 19, arrows).Air pressure: ________ psi

Fig. 20. HTF setup.9. Make the following connections to connect the HTF to the array reader:• Connect the sheath fluid line (blue fitting) to the She

Note: You may use the additional waste line provided with the system to drain to a largerwaste container. The large waste container waste must be po

15. Because the flow rate in step 14 is adjusted by altering the system pressure, it is necessaryto repeat steps 3 to 5 of Section 3.10 (Resetting Ins

Table of ContentsPageSection 1 General Information ...11.1 About This Manual ...

1 Make sure that the system has been set up according to the directions in the Millipore userguide.Hint: Make sure that the connector leading to the

3. Turn on the laboratory vacuum to maximum level (the same level that was used in the preceeding calibration procedure).4. Press on the filter plate

Section 4Care and MaintenanceTable 6. Summary of Care and Maintenance.Daily Startup, calibrate, wash between plates, shut downWeekly Sonicate needle,

Every MonthClean exterior surfaces Disconnect the instrument from AC power by turningoff the power switch on the rear of the array readerand micropla

Air intake filter Replace the air intake filter every year. Disconnect the instrument from AC power by turning off the powerswitch on the rear of the

Section 5Troubleshooting5.1 Troubleshooting Guide for Bio-Plex®200 SystemProblem Possible Cause SolutionBio-Plex Manager™ Most Likely:has detected a

Bio-Plex Manager Incorrect bead regions Compare bead regions in the assayhas detected a problem with selected in the protocol with those selected in

Pressurizing in status bar of Leak in sheath bottle or Tighten sheath cap or replace sheathsoftware. cap bottle.Sheath and waste bottle Make sure that

Bio-Plex Manager has Most likely:detected a change in sheath pressure. Sheath reservoir cap not Tighten sheath cap. Click OK. on securely Message sh

Blue power indicator light on 1. System not plugged in Turn on system, power strip.HTF is not on 2. Power strip not on Remove power cable and reconn

5.2 Troubleshooting Guide for Vacuum ManifoldMessage/Problem Causes SolutionNo Flow/no vacuum. Lid on plate Remove lid.All wells not wet, or Wet unus

5.3 Technical SupportFor technical assistance with the Bio-Plex 200 system, including all hardware and software,contact your local Bio-Rad office or,

Microplate Platform SpecificationsInput voltage range 100–240 V, ~2.25 A, 47–63 HzPhysical dimensions (W x D x H) 44 x 61 x 8 cm (17.3 x 24 x 3 in)Wei

Section 7Warranty StatementThis warranty statement may vary outside of the continental United States. Please contact yourlocal Bio-Rad office for the

Section 8Ordering Information — System AccessoriesCatalog # DescriptionGeneral System Accessories171-000050 HTF171-000055 Sheath Fluid, 20 L171-0020

CatalogNumber Product Description171-002030 Protective Shield for Sample NeedlePreventative Maintenance Items171-002032 Air Intake Filter(accessed t

CatalogNumber Product Description171-203032 MCV plate IV, for use with Bio-Plex Manager 5.0and 4.1 Software171-203050 Bio-Plex Reservoir, for use with

5. Drain the sheath fluid and waste containers.6. Rinse the waste container with 10% household bleach solution and drain.7. Remove all specimens, d

Equipment Return Information Label andDECONTAMINATION CERTIFICATEBefore Bio-Rad can accept this equipment, you must certify that it is NOT CONTAMINATE

Life Science Group Sig 1109Bio-Rad Laboratories, Inc. Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01

Section 1General Information1.1 About This ManualA Bio-Rad service engineer will install the Bio-Plex®200 system. However, the procedure is provided

21.2.1 Electrical Safety InformationWarning: This instrument must be connected to an approved power source.Warning: Do not perform any maintenance or

Caution: Removal of the array reader cover is intended for trained service personnel only. Donot attempt to operate the instrument with the cover remo

1.2.3 Mechanical Safety InformationCaution: During operation, this system contains exposed, moving parts. Risk of personal injuryis present. Keep han