Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Manuel d'utilisateur télécharger pdf (Page 3)

Table of Contents

Section 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Section 2 Kit Components . . . . . . . . . . . . . . . . . . . . . . . . .2

Section 3 Storage Conditions . . . . . . . . . . . . . . . . . . . . . . .3

Section 4 Materials and Equipment Required

(Not Provided in the Kit) . . . . . . . . . . . . . . . . . . .3

Supplies for Tissue Grinding, Disruption,

and Homogenization . . . . . . . . . . . . . . . . . . . . . . . . . . .4

Additional Equipment Required for Vacuum Format . . . .4

Section 5 Before Using the Aurum™

Total RNA Fatty and Fibrous Tissue Kit . . . . . . .5

Maximum Starting Material Amounts . . . . . . . . . . . . . . .5

Minimum Starting Material Amounts . . . . . . . . . . . . . . . .5

Reagents Used With the Aurum Total RNA

Fatty and Fibrous Tissue Kit . . . . . . . . . . . . . . . . . . . . . .7

Maintaining an RNase-Free Environment . . . . . . . . . . . .8

Sample Disruption and Homogenization . . . . . . . . . . . .9

Section 6 Vacuum Manifold Setup and Use With the

Column Adaptor Plate . . . . . . . . . . . . . . . . . . .11

Guidelines for Vacuum Format . . . . . . . . . . . . . . . . . . .11

About the Column Adapter Plate (CAP) . . . . . . . . . . . .11

Preparing the Aurum Vacuum Manifold . . . . . . . . . . . .11

Vacuum Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

Manifold Wash Setup . . . . . . . . . . . . . . . . . . . . . . . . . .13

Section 7 Vacuum Protocol . . . . . . . . . . . . . . . . . . . . . . .14

Section 8 Spin Protocol . . . . . . . . . . . . . . . . . . . . . . . . . .20

Section 9 Troubleshooting Guide . . . . . . . . . . . . . . . . . . .25

Section 10 Ordering Information . . . . . . . . . . . . . . . . . . . .32

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Bio-rad Aurum™ Total RNA Fatty and Fibrous Tissue Kit Manuel d'utilisateur Page 3