Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuel d'utilisateur télécharger pdf (Page 19)

Table 3. Products for buffer exchange.

Sample Recommended

Volume Product Use Catalog #

50–100 µl Bio-Spin 6 column Desalting proteins 732-6002

≥ 6 kD

50–100 µl Bio-Spin 30 column Desalting proteins 732-6006

≥ 30 kD

100 µl–3 ml Bio-Scale Mini P-6 Desalting proteins 732-4502

cartridge ≥ 6 kD

Up to 3 ml Econo-Pac 10DG Desalting proteins 732-2010

desalting column ≥ 6 kD

Unlimited Bio-Gel P-6DG gel Desalting proteins 150-0738

≥ 6 kD

4.2 General Purification Protocol

Ion exchange chromatography is usually performed

using increasing salt gradients or pH gradients to

elute the sample components. For best results and

to increase cartridge life, samples and buffers should

be degassed and filtered through a 0.45 µm filter.

Common buffers for cation and anion exchange

chromatography are listed in Table 4. An appropriate

starting point for purifying samples is a linear

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Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuel d'utilisateur Page 19